PKC theta Antikörper (AA 380-634)

Produktnummer ABIN7443020

Produktdetails anti-PKC theta Antikörper

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Bindungsspezifität: AA 380-634

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKC theta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Verwendungszweck: Polyclonal Antibody to Protein Kinase C Theta (PKCq)

Spezifität: The antibody is a rabbit polyclonal antibody raised against PKCq. It has been selected for its ability to recognize PKCq in immunohistochemical staining and western blotting.

Kreuzreaktivität: Maus, Ratte

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Protein Kinase C Theta (PKCq) corresdonding to Phe380~Phe634 (Accession # Q04759) with N-terminal His and GST Tag

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu PKC theta

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Andere Bezeichnung: Protein Kinase C Theta (PRKCQ Produkte)

Synonyme: PRKCT antikoerper, nPKC-theta antikoerper, A130035A12Rik antikoerper, AW494342 antikoerper, PKC-0 antikoerper, PKC-theta antikoerper, PKCtheta antikoerper, Pkcq antikoerper, protein kinase C theta antikoerper, protein kinase C eta antikoerper, protein kinase C, theta antikoerper, PRKCQ antikoerper, PRKCH antikoerper, Prkcq antikoerper

Hintergrund: PRKC-Q, PRKCQ, PRKCT, NPKC-Theta

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling