PDK1 Antikörper (AA 172-399)

Produktnummer ABIN7440905

Produktdetails anti-PDK1 Antikörper

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Bindungsspezifität: AA 172-399

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Verwendungszweck: Polyclonal Antibody to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1)

Spezifität: The antibody is a rabbit polyclonal antibody raised against PDK1. It has been selected for its ability to recognize PDK1 in immunohistochemical staining and western blotting.

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1) corresdonding to Val172~Pro399 with N-terminal His Tag

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western blotting: 0.5-2 μg/mL

1:250-1000 Immunohistochemistry: 5-20 μg/mL

1:25-100 Immunocytochemistry: 5-20 μg/mL

1:25-100 Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu PDK1

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Andere Bezeichnung: Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1 Produkte)

Synonyme: ATP5A antikoerper, B830012B01 antikoerper, D530020C15Rik antikoerper, si:zfos-979f1.3 antikoerper, 3-phosphoinositide dependent protein kinase-1 antikoerper, pyruvate dehydrogenase kinase 1 antikoerper, ATP synthase, H+ transporting, mitochondrial Fo complex subunit C2 (subunit 9) antikoerper, pyruvate dehydrogenase kinase, isoenzyme 1 antikoerper, pyruvate dehydrogenase kinase, isozyme 1 antikoerper, pyruvate dehydrogenase kinase 1 L homeolog antikoerper, Pdpk1 antikoerper, PDK1 antikoerper, ATP5G2 antikoerper, Pdk1 antikoerper, pdk1 antikoerper, pdk1.L antikoerper

Hintergrund: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial

Pathways: PI3K-Akt Signalweg, RTK Signalweg, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Warburg Effekt