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His Tag Antikörper

Name: His Tag Antikörper
Brand: antibodies-online
SKU: ABIN7250827
Price: 259 EUR
Availability: InStock

WB, IP, IF Wirt: Maus Monoclonal 5I8 unconjugated

Western Blot analysis of 2 μg His fusion protein using His-Tag Monoclonal Antibody at dilution of 1) 1:5000 2) 1:10000. (His Tag Antikörper)

Immunofluorescence analysis of 293 cells transfected with a His tag protein tissue using His-Tag Monoclonal Antibody at dilution of 1:1000. (His Tag Antikörper)

2 Abbildungen

Produktnummer ABIN7250827

Datenblatt (PDF)

Target Alle His Tag Antikörper anzeigen

His Tag

Reaktivität

Bitte anfragen
Wirt
89

60

11

9

5

4

1

1
Maus
Passende Sekundärantikörper
Klonalität
91

76

10
Monoklonal
Konjugat
76

22

13

13

4

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser His Tag Antikörper ist unkonjugiert
Applikation
138

89

36

25

24

16

14

14

13

13

13

7

7

5

5

4

4

4

3

3

2

2

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Produktmerkmale

Tag Antibody

Aufreinigung

Protein A purification

Immunogen

Synthetic Peptide

Klon

5I8

Isotyp

IgG

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Applikationshinweise

WB 1:5000-1:10000, IF 1:500-1:2000, IP 1:100-1:300

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 mg/mL

Buffer

PBS with 0.02 % sodium azide and 50 % glycerol pH 7.4.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C

Informationen zur Lagerung

Store at -20°C. Avoid freeze / thaw cycles.
Target

His Tag

Andere Bezeichnung

His-Tag (His Tag Produkte)

Substanzklasse

Tag

Hintergrund

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. The His tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells.