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MBP Tag Antikörper

WB Wirt: Maus Monoclonal 8K2 unconjugated
Produktnummer ABIN7250836
  • Target
    MBP Tag
    Wirt
    • 12
    • 5
    Maus
    Klonalität
    • 11
    • 5
    Monoklonal
    Konjugat
    • 15
    • 1
    • 1
    Unkonjugiert
    Applikation
    • 15
    • 8
    • 7
    • 5
    • 3
    • 1
    • 1
    Western Blotting (WB)
    Produktmerkmale
    Tag Antibody
    Aufreinigung
    Protein A purification
    Immunogen
    Recombinant Protein
    Klon
    8K2
    Isotyp
    IgG
  • Applikationshinweise
    WB 1:5000-1:10000
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS with 0.02 % sodium azide and 50 % glycerol pH 7.4.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at -20°C. Avoid freeze / thaw cycles.
  • Target
    MBP Tag
    Andere Bezeichnung
    MBP-Tag
    Substanzklasse
    Tag
    Hintergrund
    Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Maltose binding protein(MBP) is the 370 amino acid product of the E.coli mal E gene. MBP is a useful affinity tag that can increase the expression level and solubility of the resulting tagged protein. The MBP tag also promotes proper folding of the attached protein. Plasmid vectors have been constructed utilizing the MBP domain that allow the synthesis of high levels of MBP-fusion proteins that can be purified in a one step procedure by affinity chromatography cross linked amylose resin. Once bound to amylose, the MBP protein can then be separated from the target protein by cleavage by coagulation Factor Xa at a specific four residue site.
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