Rekombinanter EDAR Antikörper

Produktnummer ABIN7072395

Produktdetails anti-EDAR Antikörper

Target: EDAR (Ectodysplasin A Receptor (EDAR))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser EDAR Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Surface Plasmon Resonance (SPR), Agonist (Agon)

Verwendungszweck: Anti-EDAR [EDAR12], Rabbit IgG, kappa

Spezifität: EDAR12 recognises and binds to CRD1 of the extracellular domain of mouse EDAR. EDAR3 cross-reacts with EDAR derived from human, dog, rat and chicken when EDAR is fused to the glycosylphosphatidylinositol anchor of TRAILR3. EDAR is the receptor for the TNF family ligand EDA1, which is a type II transmembrane protein possessing a collagen-like domain and a C-terminal TNF-homology domain. EDAR is important for the correct development of skin appendages including hair, teeth and eccrine sweat glands. LoF mutations in the Eda gene is known to cause XLHED (X-linked hypohidrotic ectodermal dysplasia), and results in abnormal development.

Kreuzreaktivität: Huhn, Hund, Maus, Ratte

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: EDAR12 was prepared by immunixzing female OVE1B mice (with the Edar gene deleted) subcutaneously with mouse EDAR-Fc and positive hybridoma clones were screened for binding mEDAR by ELISA.

Klon: EDAR12

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: EDAR12 can be used in surrogate reporter assay in which Fas-sensitive cells were transfected with a construct formed by fusing the ectodomain of mouse or human EDAR to the intracellular domain of Fas. Binding of EDAR12 resulted in the induction of apoptosis in these cells, confirming the agonisitc activity of the antibody. (EC50 (dose required to kill half of EDAR:Fas-expressing Jurkat cells) hEDAR:Fas ~ 5 ng/mL, EC50 mEDAR:Fas ~ 10 ng/mL - both were less active than DEA1-Fc). Tail-hair formation was also rescued after administration of EDAR3 to newborn EDA-deficient Tabby mice, with 0.35 mg/kg required for half-maximal tail-hair reversion. EDAR123 can also be used in ELISA and as a Fab fragment (generated by ficin digestion) in SPR experiments. EDAR can also be recognized by EDAR12 under both reducing (+DTT) and non-reducing conditions.

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu EDAR

Target: EDAR (Ectodysplasin A Receptor (EDAR))

Andere Bezeichnung: EDAR (EDAR Produkte)

Hintergrund: Ectodysplasin A receptor, Tumor necrosis factor receptor superfamily member EDAR, Anhidrotic ectodysplasin receptor 1, Downless, Ectodermal dysplasia receptor, Ectodysplasin-A receptor

UniProt: Q9R187

Pathways: Tube Formation, Ubiquitin Proteasome Pathway