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Rekombinanter MHC Class II Antikörper

MHC2 Reaktivität: Maus, Ratte FACS, IHC, IP Wirt: Kaninchen Chimeric P7-7 unconjugated Recombinant Antibody
Produktnummer ABIN7072422
  • Target Alle MHC Class II (MHC2) Antikörper anzeigen
    MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
    Antikörpertyp
    Recombinant Antibody
    Reaktivität
    • 48
    • 45
    • 15
    • 9
    • 9
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    Maus, Ratte
    Wirt
    • 46
    • 28
    • 24
    Kaninchen
    Klonalität
    • 78
    • 19
    • 1
    Chimeric
    Konjugat
    • 31
    • 15
    • 10
    • 10
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser MHC Class II Antikörper ist unkonjugiert
    Applikation
    • 70
    • 42
    • 22
    • 22
    • 20
    • 13
    • 13
    • 9
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Verwendungszweck
    Anti-MHC II [P7/7], Rabbit IgG, kappa
    Spezifität
    This antibody recognises the rat RT1Bu/Du MHC class II antigen, and cross-reacts with haplotypes c, d, l and n. This antibody stains MHC class II molecules in a number of rat strains, including PVG, F334, LEW, and BN. However, this antibody is not reactive with RT1a haplotype rats, including Wistar rats. This antibody also cross-reacts with murine I-A and I-E molecules of all haplotypes tested, including H-2b, d, f and k, so can be used in all mouse strains.
    Kreuzreaktivität
    Maus
    Produktmerkmale

    Original Species of Ab: Rat

    Original Format of Ab: IgG2b

    Aufreinigung
    Protein A affinity purified
    Immunogen
    This antibody was raised by immunising DA rats with PVG.R8 rat spleen cells. Splenocytes from immunised rats were then isolated, and fused to the myeloma line NSO/u to generate stable hybridomas.
    Klon
    P7-7
    Isotyp
    IgG kappa
    Top Product
    Discover our top product MHC2 Primärantikörper
  • Applikationshinweise
    This antibody reacts with murine MHC class II I-A and I-E molecules, as confirmed by two-dimensional gel electrophoresis of Ia immunoprecipitates from metabolically labelled murine lymphoma cells (Momburg et al, 1986). Cross-blocking studies with anti-Ia β-chain MAbs indicate that P7/7 binds to the β-chain of murine Ia (Momburg et al, 1986). This antibody has been used to stain acetone-fixed mouse pancreas sections to study lymphocyte infiltration (Signore et al, 1987, Signore et al, 1989) and ethanol-fixed mouse brain sections (McMenamin et al, 1998). In flow cytometric analysis, this antibody has been employed to identify MHC class II-expressing murine cells (Hayball & Lake, 2004, Heath et al, 1991), and to analyse the surface phenotype of murine N9 microglial cells (Dimayuga et al, 2005). This antibody has been used in immunofluorescence analysis of acetone-fixed thymus sections to identify MHC-II positive and negative medullae (Aschenbrenner et al, 2007).
    Kommentare

    This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    PBS with 0.02 % Proclin 300.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Target
    MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
    Andere Bezeichnung
    MHC II (MHC2 Produkte)
    Hintergrund
    P7.7, I-A, Ia, I-E, MHC-II, MHC Class II
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