Rekombinanter MHC Class II Antikörper
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- Target Alle MHC Class II (MHC2) Antikörper anzeigen
- MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
- Antikörpertyp
- Recombinant Antibody
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Reaktivität
- Maus, Ratte
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Wirt
- Kaninchen
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Klonalität
- Chimeric
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Konjugat
- Dieser MHC Class II Antikörper ist unkonjugiert
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Applikation
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Verwendungszweck
- Anti-MHC II [P7/7], Rabbit IgG, kappa
- Spezifität
- This antibody recognises the rat RT1Bu/Du MHC class II antigen, and cross-reacts with haplotypes c, d, l and n. This antibody stains MHC class II molecules in a number of rat strains, including PVG, F334, LEW, and BN. However, this antibody is not reactive with RT1a haplotype rats, including Wistar rats. This antibody also cross-reacts with murine I-A and I-E molecules of all haplotypes tested, including H-2b, d, f and k, so can be used in all mouse strains.
- Kreuzreaktivität
- Maus
- Produktmerkmale
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Original Species of Ab: Rat
Original Format of Ab: IgG2b
- Aufreinigung
- Protein A affinity purified
- Immunogen
- This antibody was raised by immunising DA rats with PVG.R8 rat spleen cells. Splenocytes from immunised rats were then isolated, and fused to the myeloma line NSO/u to generate stable hybridomas.
- Klon
- P7-7
- Isotyp
- IgG kappa
- Top Product
- Discover our top product MHC2 Primärantikörper
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- Applikationshinweise
- This antibody reacts with murine MHC class II I-A and I-E molecules, as confirmed by two-dimensional gel electrophoresis of Ia immunoprecipitates from metabolically labelled murine lymphoma cells (Momburg et al, 1986). Cross-blocking studies with anti-Ia β-chain MAbs indicate that P7/7 binds to the β-chain of murine Ia (Momburg et al, 1986). This antibody has been used to stain acetone-fixed mouse pancreas sections to study lymphocyte infiltration (Signore et al, 1987, Signore et al, 1989) and ethanol-fixed mouse brain sections (McMenamin et al, 1998). In flow cytometric analysis, this antibody has been employed to identify MHC class II-expressing murine cells (Hayball & Lake, 2004, Heath et al, 1991), and to analyse the surface phenotype of murine N9 microglial cells (Dimayuga et al, 2005). This antibody has been used in immunofluorescence analysis of acetone-fixed thymus sections to identify MHC-II positive and negative medullae (Aschenbrenner et al, 2007).
- Kommentare
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This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % Proclin 300.
- Konservierungsmittel
- ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Target
- MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))
- Andere Bezeichnung
- MHC II (MHC2 Produkte)
- Hintergrund
- P7.7, I-A, Ia, I-E, MHC-II, MHC Class II
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