Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Rekombinanter CD131 Antikörper

CSF2RB Reaktivität: Human IHC, IP, BP, Crys, Func Wirt: Kaninchen Chimeric BION-1 unconjugated Recombinant Antibody
Produktnummer ABIN7072454
  • Target Alle CD131 (CSF2RB) Antikörper anzeigen
    CD131 (CSF2RB) (Colony Stimulating Factor 2 Receptor, Beta (CSF2RB))
    Antikörpertyp
    Recombinant Antibody
    Reaktivität
    • 69
    • 10
    • 6
    • 1
    Human
    Wirt
    • 57
    • 12
    • 2
    Kaninchen
    Klonalität
    • 58
    • 13
    Chimeric
    Konjugat
    • 40
    • 6
    • 5
    • 5
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser CD131 Antikörper ist unkonjugiert
    Applikation
    • 57
    • 38
    • 20
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunoprecipitation (IP), Blocking Peptide (BP), Crystallization (Crys), Functional Studies (Func)
    Verwendungszweck
    Anti-CD131 [BION-1], Rabbit IgG, kappa
    Spezifität
    Binds to domain 4 of the common beta-chain and recognises the same binding site used by IL-5, GM-CSF and IL-3, or binds to an epitope in close proximity to it. Residues M363, R364, E366 (B-C loop) and R418 (F-G loop) in the common beta-chain are important for BION-1 binding.
    Produktmerkmale

    Original Species of Ab: Mouse

    Original Format of Ab: IgG

    Aufreinigung
    Protein A affinity purified
    Immunogen
    BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method. MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column.
    Klon
    BION-1
    Isotyp
    IgG kappa
    Top Product
    Discover our top product CSF2RB Primärantikörper
  • Applikationshinweise
    BION-1 has been shown the block eosinophil production, survival, and activation stimulated by IL-5 as well as by GM-CSF and IL-3, thereby influencing inflammation (Sun et al., 1999, PMID: 10477723). Further, BION-1 has been used in immunohistochemistry experiments in order to examine the presence of GM-CSF-receptor protein in human 8-cell embryos and blastocysts (Sjoblom et al., 2002, PMID: 12444058). In addition, a fab fragment derived from BION-1 has been used in the crystallisation of the common beta-chain to analyse the structure of the activation domain of the GM-CSF/IL-3/IL-5 receptor (Rossjohn et al., 2000, PMID: 10753826).
    Kommentare

    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    PBS with 0.02 % Proclin 300.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Target
    CD131 (CSF2RB) (Colony Stimulating Factor 2 Receptor, Beta (CSF2RB))
    Andere Bezeichnung
    CD131 (CSF2RB Produkte)
    Hintergrund
    CSF2RB, CD131, IL3RB, IL-3R common beta subunit, IL5RB, colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage), SMDP5, Common Beta-Chain, CDw131, cytokine receptor common subunit beta, beta-c, CD131, Beta Common Chain
    UniProt
    P32927
    Pathways
    JAK-STAT Signalweg
Sie sind hier:
Kundenservice