Rekombinanter TIGIT Antikörper

Produktnummer ABIN7072458

Produktdetails anti-TIGIT Antikörper

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser TIGIT Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Agonist (Agon)

Verwendungszweck: Anti-TIGIT [4D4], Rabbit IgG, kappa

Spezifität: 4D4 specifically recognises TIGIT.

Produktmerkmale:

Original Species of Ab: Hamster, Armenian

Original Format of Ab: IgG

Aufreinigung: Protein A affinity purified

Immunogen: Armenian hamsters were immunized with recombinant mouse TIGIT tetramers by a combination of s.c. and food pad immunization and booster injections. Draining lymph nodes were fused with Sp2/0-Ag14 cells, selected in HAT (hypoxanthine/aminopterin/thymidine) medium and supernatants were screened for specificity by ELISA and flow cytometry using TIGIT-transfectants.

Klon: 4D4

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: 4D4 is an agonistic anti-TIGIT antibody, and addition of plate bound 4D4 to anti-CD3/anti-CD28 stimulated T cells was shown to inhibit their proliferation (Joller 2011). Further, 4D4 has been used in order to identify genes that are downstream of TIGIT signaling in Tregs, as TIGIT engagement might modulate the Treg phenotype in tumor tissue (Liu 2016).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu TIGIT

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Andere Bezeichnung: TIGIT (TIGIT Produkte)

Hintergrund: VSIG9, T Cell Immunoreceptor With Ig And ITIM Domains, V-Set And Immunoglobulin Domain Containing Protein 9, V-Set And Transmembrane Domain Containing Protein 3, VSTM3, T Cell Immunoreceptor With Ig And ITIM Domains, Washington University Cell Adhesion Molecule, WUCAM

UniProt: P86176

Pathways: Cancer Immune Checkpoints