Rekombinanter CD70 Antikörper
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- Target Alle CD70 Antikörper anzeigen
- CD70 (CD70 Molecule (CD70))
- Antikörpertyp
- Recombinant Antibody
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Reaktivität
- Maus
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Wirt
- Kaninchen
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Klonalität
- Chimeric
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Konjugat
- Dieser CD70 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Blocking Peptide (BP)
- Verwendungszweck
- Anti-CD70 [TAN1-6], Rabbit IgG, kappa
- Spezifität
- This antibody is specific for murine CD70, a 30-33 kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.
- Produktmerkmale
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Original Species of Ab: Rat
Original Format of Ab: IgG2a
- Aufreinigung
- Protein A affinity purified
- Immunogen
- This antibody was raised by immunising LOU rats with soluble recombinant CD70:Fc fusion protein
- Klon
- TAN1-6
- Isotyp
- IgG kappa
- Top Product
- Discover our top product CD70 Primärantikörper
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- Applikationshinweise
- This antibody has been used in various FACS analyses to reveal a critical role for CD70 in CD8 T cell priming by CD40-licensed antigen-presenting cells (Taraban et al, 2004), to demonstrate the differential requirement for CD70 and CD80/CD86 in dendritic cell-mediated activation of tumor-tolerized CD8 T cells (Bak et al, 2012), to examine how temporal changes in dendritic cell subsets, cross-priming and costimulation via CD70 control CD8(+) T cell responses to influenza (Ballesteros-Tato et al, 2010), and to show that CD70 is downregulated by interaction with CD27 (Kuka et al, 2013). In addition, this antibody has been used in vivo to block the CD70-CD27 interaction as part of the two studies examining how CD70 is critically required for the priming of CD8+ T cells (Taraban et al, 2004, Taraban et al, 2006).
- Kommentare
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This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1 mg/mL
- Buffer
- PBS with 0.02 % Proclin 300.
- Konservierungsmittel
- ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Target
- CD70 (CD70 Molecule (CD70))
- Andere Bezeichnung
- CD70 (CD70 Produkte)
- Hintergrund
- TNFSF7, CD27 ligand, CD27-L, Cd27l, CD27LG, CD70 antigen, Ki-24 antigen, Tnfsf7, tumor necrosis factor (ligand) superfamily, member 7, Tumor necrosis factor ligand superfamily member 7
- UniProt
- O55237
- Pathways
- Cancer Immune Checkpoints
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