Rekombinanter TIGIT Antikörper

Produktnummer ABIN7072547

Produktdetails anti-TIGIT Antikörper

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser TIGIT Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS), Blocking Peptide (BP)

Verwendungszweck: Anti-TIGIT [1B4], Rabbit IgG, lambda

Spezifität: This antibody is specific for murine TIGIT.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised by immunising TIGIT-/- mice with recombinant murine TIGIT tetramers.

Klon: 1B4

Isotyp: IgG lambda

Anwendungsinformationen

Applikationshinweise: The binding specificity of this antibody for TIGIT has been confirmed in ELISA analysis and by flow cytometry using P815 cells transfected with mouse TIGIT and activated mouse splenocytes (Dixon et al, 2018). This antibody also specifically stains activated primary wild-type T cells (Dixon et al, 2018). This antibody fully blocks CD155 binding to TIGIT, and acts as a TIGIT blocking antibody in vivo. Administration of this antibody increases experimental autoimmune encephalomyelitis (EAE) severity, and retards the growth of established MC38 colon carcinoma and GL261 glioblastoma (Dixon et al, 2018). This antibody synergises with anti-PD-1 blocking antibodies to achieve complete tumour regression in colon carcinoma models (Dixon et al, 2018). The original mouse IgG1 antibody does not deplete TIGIT-expressing CD4+ or CD8+ T cells in vivo, nor affect T cell proliferation in vitro (Dixon et al, 2018).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu TIGIT

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Andere Bezeichnung: TIGIT (TIGIT Produkte)

Hintergrund: VSTM3, T cell Ig and ITIM domain, T cell immunoreceptor with Ig and ITIM domains, V-set and transmembrane domain-containing protein 3, WUCAM

UniProt: P86176

Pathways: Cancer Immune Checkpoints