Rekombinanter MS4A4A Antikörper

Produktnummer ABIN7072576

Produktdetails anti-MS4A4A Antikörper

Target: MS4A4A (Membrane-Spanning 4-Domains, Subfamily A, Member 4 (MS4A4A))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser MS4A4A Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP)

Verwendungszweck: Anti-MS4A4A [5C12], Rabbit IgG, lambda

Spezifität: This antibody is specific for human extracellular MS4A4A. 5C12 mAb only partially cross-blocked either 4H2 (Ab01373) and 3F2 (Ab01375) and therefore probably detects a unique or overlapping epitope (Sanyal et al., 2017, pmid: 28303902). MS4A (membrane-spanning 4-domain family, subfamily A) is a large family of proteins that includes at least 26 members in mouse and humans. Flanked by amino- and carboxyl- cytoplasmic regions, MS4A family members contain four highly conserved transmembrane domains connected by short extracellular and cytoplasmic loops. MS4A4A is likely to be a component of oligomeric cell surface complexes involved in signal transduction in diverse cell lineages.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was generated by immunizing C57BL/6 mice against mouse cell line 300-19 expressing transfected human membrane-spanning 4-domains subfamily A member 4A (MS4A4A)-GFP.

Klon: 5C12

Isotyp: IgG lambda

Anwendungsinformationen

Applikationshinweise: 5C12 is recommended for the detection and analysis of expression of MS4A4A. Importantly, MS4A4A has been shown to be a marker for M2 macrophages and plasma cells and to be overexpressed in multiple myelomas, suggesting a potential role in targeting alternatively activated macrophages such as tumor-associated macrophages, and in the treatment of multiple myeloma and mantle cell lymphoma (Sanyal et al., 2017, pmid: 28303902). The antibody 5C12 can be successfully used for the detection of MS4A4A-expressing cells via flow cytometry (Sanyal et al., 2017, pmid: 28303902). Finally, specificity of this antibody was additionally confirmed via immunoprecipitation of lysates of BJAB MS4A4A-HA cells (Sanyal et al., 2017, pmid: 28303902).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu MS4A4A

Target: MS4A4A (Membrane-Spanning 4-Domains, Subfamily A, Member 4 (MS4A4A))

Andere Bezeichnung: MS4A4A (MS4A4A Produkte)

Hintergrund: Four-span transmembrane protein 1, human membrane-spanning 4-domains subfamily A member 4A, CD20 antigen-like 1, (MS4A4A)-GFP, MS4A4, 4SPAN1, MS4A

UniProt: Q96JQ5