Rekombinanter CD53 Antikörper

Produktnummer ABIN7072601

Produktdetails anti-CD53 Antikörper

Target: CD53

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD53 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Verwendungszweck: Anti-CD53 [HD77], Rabbit IgG, kappa

Spezifität: This antibody is specific for the extracytoplasmic part of human CD53, a pan-leukocyte antigen.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised by immunising mice with human leukemia cells.

Klon: HD77

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody has applications in the detection of normal and neoplastic white blood cells. This antibody has been used to stain tumour and mononuclear cells for CD53 expression in flow cytometric analysis of samples from ovarian carcinoma patients (Marme et al, 2002), and cells of the colon carcinoma line HT29 (Koretz et al, 1992). In immunohistochemical analysis of frozen tissue sections, this antibody has been shown to react with osteoclasts and osteoclast-like giant cells in malignant fibrous histiocytoma and in benign giant cell tumor of tendon sheath (Mechtersheimer et al, 1990). This antibody has been used to re-immunoprecipitate CD53 from PKCα immunoprecipiates (Zhang et al, 2001).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD53

Target: CD53

Andere Bezeichnung: CD53 (CD53 Produkte)

Hintergrund: TSPAN-25, Leukocyte surface antigen CD53, Tetraspanin-25, MOX44, TSPAN25

UniProt: P19397