Pol II Antikörper
-
- Target
- Pol II
- Reaktivität
- Human
- Wirt
- Maus
- Klonalität
- Monoklonal
- Applikation
- Immunofluorescence (IF), Western Blotting (WB)
- Kreuzreaktivität
- Human
- Aufreinigung
- Purified by Protein G.
- Immunogen
- Monoclonal antibody raised in mouse against the YSPTSPS repeat in the B1 subunit of RNA polymerase II
- Isotyp
- IgG1
-
- Applikationshinweise
-
WB 1:300-5000
IF() - Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Konzentration
- 1 μg/μL
- Buffer
- PBS with 0.05 % sodium azide.
- Konservierungsmittel
- ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at -20°C for 12 months.
- Haltbarkeit
- 12 months
-
- Target
- Pol II
- Synonyme
- 152117_at antikoerper, BG:DS00941.10 antikoerper, CG7885 antikoerper, D-rpII33 antikoerper, Dmel\\CG7885 antikoerper, Pol II antikoerper, Pol II RPII33 antikoerper, PolII antikoerper, PolIIo antikoerper, RNA Pol II antikoerper, RNA pol II antikoerper, RNA polII antikoerper, RNAP antikoerper, RPB3 antikoerper, Rbp3 antikoerper, Rpb3 antikoerper, Rpll33 antikoerper, br17 antikoerper, l(2)34Dg antikoerper, l(2)br17 antikoerper, l(2)k05605 antikoerper, l34Dg antikoerper, pol II antikoerper, polII antikoerper, rpII33 antikoerper, CG3180 antikoerper, DmRP140 antikoerper, Dmel\\CG3180 antikoerper, H5 antikoerper, II antikoerper, Pol IIc antikoerper, PolIIc antikoerper, RNA Pol II 140 antikoerper, RNA Pol IIc antikoerper, RNAP II antikoerper, RNApolII antikoerper, RP140 antikoerper, RPB2_DROME antikoerper, RPII140 antikoerper, RpII140[wimp] antikoerper, Rpb2 antikoerper, Rpll140 antikoerper, l(3)RplII140 antikoerper, rpII140/wimp antikoerper, wimp antikoerper, CG3284 antikoerper, Dmel\\CG3284 antikoerper, Pol-II antikoerper, RPII15 antikoerper, RpII215 antikoerper, dRPB9 antikoerper, l(3)88Be antikoerper, l(3)Z23 antikoerper, BcDNA:RH21608 antikoerper, CG1163 antikoerper, Dm6 antikoerper, Dmel\\CG1163 antikoerper, RPB6_DROME antikoerper, RPII18 antikoerper, RpABC14 antikoerper, Rpll18 antikoerper, DNA-directed RNA polymerase II subunit RPB1 antikoerper, RNA polymerase II large subunit Rpb1 antikoerper, RNA polymerase II 33kD subunit antikoerper, RNA polymerase II 140kD subunit antikoerper, RNA polymerase II 15kD subunit antikoerper, RNA polymerase II 18kD subunit antikoerper, EDI_169920 antikoerper, CMU_000800 antikoerper, SJAG_01713 antikoerper, MGYG_01527 antikoerper, RpII33 antikoerper, RpII140 antikoerper, RpII15 antikoerper, RpII18 antikoerper
- Hintergrund
-
Synonyms: POLR2A, RPB1, POLR2, RPOL2
Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (By similarity). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression
- Gen-ID
- 5430
- UniProt
- P24928
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