RTF1 Antikörper

Produktnummer ABIN7118711

Produktdetails anti-RTF1 Antikörper

Target: RTF1 (RNA polymerase-associated protein RTF1 homolog (RTF1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RTF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Aufreinigung: Immunogen affinity purified

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: Rtf1, Paf1/RNA polymerase II complex component, homolog(S. cerevisiae)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000, IHC: 1:20-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Haltbarkeit: 12 months

Details zu RTF1

Target: RTF1 (RNA polymerase-associated protein RTF1 homolog (RTF1))

Andere Bezeichnung: RTF1 (RTF1 Produkte)

Synonyme: CG10955 antikoerper, Dmel\\CG10955 antikoerper, dRtf1 antikoerper, MGC89486 antikoerper, RTF1 antikoerper, fb33g03 antikoerper, wu:fa04h07 antikoerper, wu:fb33g03 antikoerper, GTL7 antikoerper, KIAA0252 antikoerper, 2900005O08Rik antikoerper, 6530416A09Rik antikoerper, AI853581 antikoerper, AU043144 antikoerper, AW553985 antikoerper, Gtl7 antikoerper, cbp82 antikoerper, CG10955 gene product from transcript CG10955-RB antikoerper, RTF1 homolog, Paf1/RNA polymerase II complex component antikoerper, RTF1 homolog, Paf1/RNA polymerase II complex component L homeolog antikoerper, RTF1, Paf1/RNA polymerase II complex component antikoerper, Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae) antikoerper, Rtf1 antikoerper, rtf1 antikoerper, RTF1 antikoerper, rtf1.L antikoerper

Hintergrund: Synonyms:KIAA0252 Background:Component of the PAF1 complex(PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'-and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1, it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4'(H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B(H2BK120ub1), UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single-stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4'(H3K4me3) on genes involved in stem cell pluripotency, this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex(By similarity).

Molekulargewicht: 100 kDa

Gen-ID: 23168

UniProt: Q92541

Pathways: Stem Cell Maintenance