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Layilin Antikörper (AA 281-382)

Name: Layilin Antikörper (AA 281-382)
SKU: ABIN707756
Price: 384.62 EUR
Availability: InStock

LAYN Reaktivität: Maus WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded mouse placenta, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with (Layilin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin for 40 minutes and DAPI staining of the nuclei. (Layilin Antikörper (AA 281-382))

IF (p)

Paraformaldehyde-fixed, paraffin embedded Mouse small intestine tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with Layilin Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (Layilin Antikörper (AA 281-382))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse intestines, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with (Layilin) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin for 40 minutes and DAPI staining of the nuclei. (Layilin Antikörper (AA 281-382))

IF (p)

3 Abbildungen

Produktnummer ABIN707756

Datenblatt (PDF)

Target Alle Layilin (LAYN) Antikörper anzeigen

Layilin (LAYN)
Bindungsspezifität
4

3

2

2

2

2

2

1
AA 281-382
Reaktivität
25

11

5

2

1

1

1

1

1
Maus
Wirt
18

7
Kaninchen
Passende Sekundärantikörper
Klonalität
20

5
Polyklonal
Konjugat
15

2

1

1

1

1

1

1

1

1
Dieser Layilin Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität

Maus

Homologie

Human,Rat,Dog,Cow,Horse,Chicken,Rabbit

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human Layilin

Isotyp

IgG

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LAYN Reaktivität: Human WB Wirt: Maus Polyclonal unconjugated

anti-Layilin (LAYN) (Internal Region) antibody
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anti-Layilin (LAYN) (AA 262-382) antibody
LAYN Reaktivität: Human ELISA, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Layilin (LAYN) (AA 41-90) antibody
LAYN Reaktivität: Human, Maus, Ratte, Meerschweinchen, Schwein WB Wirt: Kaninchen Polyclonal unconjugated

anti-Layilin (LAYN) (N-Term) antibody
LAYN Reaktivität: Human WB Wirt: Kaninchen Polyclonal unconjugated

anti-Layilin (LAYN) (Internal Region) antibody
LAYN Reaktivität: Human, Maus, Ratte WB, ELISA, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Layilin (LAYN) (AA 262-382) antibody (HRP)
LAYN Reaktivität: Human ELISA Wirt: Kaninchen Polyclonal HRP

anti-Layilin (LAYN) (AA 262-382) antibody (FITC)
LAYN Reaktivität: Human Wirt: Kaninchen Polyclonal FITC

anti-Layilin (LAYN) (AA 262-382) antibody (Biotin)
LAYN Reaktivität: Human ELISA Wirt: Kaninchen Polyclonal Biotin

Applikationshinweise

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Target

Layilin (LAYN)

Andere Bezeichnung

Layilin (LAYN Produkte)

Synonyme

LAYN antikoerper, si:dkey-27p23.2 antikoerper, E030012M19Rik antikoerper, Gm511 antikoerper, RGD1564444 antikoerper, layilin antikoerper, layilin a antikoerper, LAYN antikoerper, layna antikoerper, Layn antikoerper

Hintergrund

Synonyms: Layn, E030012M19Rik, Gm511.
Background: LAYN is integral membrane protein, and a hyaluronate receptor. It has been shown to bind specifically to hyaluronan but not to other glycosaminoglycans. It is a recently characterized transmembrane protein with homology to C type lectins, and is a membrane binding site for talin in peripheral ruffles of spreading cells. LAYN may have a role in cell adhesion and motility.

Gen-ID

143903