Rekombinanter Podoplanin Antikörper (AA 1-100)

Produktnummer ABIN7504147

Produktdetails anti-Podoplanin Antikörper

Target: Podoplanin (PDPN)

Antikörpertyp: Recombinant Antibody

Bindungsspezifität: AA 1-100

Reaktivität: Synthetic

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Podoplanin Antikörper ist unkonjugiert

Applikation: ELISA, Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: It recognizes a muco-protein of 38-43 kDa, which is identified as Podoplanin (PDPN). It localizes in stromal cells of peripheral lymphoid tissue and thymic epithelial cells. As a regulator of the lymphatic endothelium, podoplanin probably plays a role in maintaining the unique shape of podocytes. It is selectively expressed in lymphatic endotheliumas well as lymphoangiomas,Kaposi sarcomas,and in a subset of angiosarcomas with probable lymphatic differentiation. Recent studies have also shown podoplanin to be a highly sensitive and relatively specific marker for epithelioid mesothelioma. Therefore, it can be used in a panel to distinguish mesotheliomas or mesothelial cells from pulmonary carcinomas.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Synthetic peptide corresponding to residues within aa1-100 of Podoplanin

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: Human tonsil, cervical or lung carcinoma tissues.

Known Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Haltbarkeit: 24 months

Details zu Podoplanin

Target: Podoplanin (PDPN)

Andere Bezeichnung: PDPN (PDPN Produkte)

Hintergrund: Aggrus, Glycoprotein 36 KD, Glycoprotein 36, gp36, GP38, GP40, HT1A1, hT1alpha1, hT1alpha2, Lung type I cell membrane associated glycoprotein, Lung type I cell membrane associated glycoprotein T1A 2, OTS8,Podoplanin (PDPN) (Lymphatic Endothelial & Mesothelial Marker)
Cellular localisation: Cell Surface

Molekulargewicht: 17kDa

Gen-ID: 10630, 468675

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport