UBE2C Antikörper

Produktnummer ABIN7504126

Produktdetails anti-UBE2C Antikörper

Target: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser UBE2C Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Spezifität: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ubconjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2C, also designated UBCH10 in human, is an E2 ubiquitin conjugating enzyme for the anaphase promoting complex (APC), which coordinates mitosis and G1 by sequentially promoting the degradation of key cell-cycle regulators. UBE2C is overexpressed in many different types of cancers and may be a potential therapeutic target.

Kreuzreaktivität (Details): Human

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length UBE2C protein

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa, Jurkat or A431 cells. Human tonsil or placenta tissue.

Known Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Haltbarkeit: 24 months

Details zu UBE2C

Target: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Andere Bezeichnung: UBE2C (UBE2C Produkte)

Synonyme: LOC458287 antikoerper, UBCH10 antikoerper, dJ447F3.2 antikoerper, 1110015A16Rik antikoerper, D2Ertd695e antikoerper, im:7137135 antikoerper, zgc:123190 antikoerper, ubiquitin conjugating enzyme E2 C antikoerper, ubiquitin-conjugating enzyme E2C antikoerper, ubiquitin conjugating enzyme E2 C L homeolog antikoerper, ubiquitin conjugating enzyme E2 C S homeolog antikoerper, UBE2C antikoerper, NAEGRDRAFT_82946 antikoerper, ube2c.L antikoerper, Ube2c antikoerper, ube2c.S antikoerper, ube2c antikoerper

Hintergrund: Cyclin selective ubiquitin carrier protein, mitotic specific ubiquitin conjugating enzyme, UB E2C, UBCH10, Ubiquitin protein ligase C,Ubiquitin-conjugating Enzyme E2C / UBCH10
Cellular localisation: Cytoplasm.

Molekulargewicht: 20kDa

Gen-ID: 11065, 93002

UniProt: O00762

Pathways: Ubiquitin Proteasome Pathway