Rekombinanter EPX Antikörper

Produktnummer ABIN7127087

Produktdetails anti-EPX Antikörper

Target: EPX (Eosinophil Peroxidase (EPX))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EPX Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human eosinophils from a patient with hypereosinophilic syndrome

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 cells. Tonsil

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu EPX

Target: EPX (Eosinophil Peroxidase (EPX))

Andere Bezeichnung: EPX (EPX Produkte)

Synonyme: EPX antikoerper, pmr-1 antikoerper, EPO antikoerper, EPP antikoerper, EPX-PEN antikoerper, eosinophil peroxidase antikoerper, eosinophil peroxidase L homeolog antikoerper, LOC788751 antikoerper, EPX antikoerper, epx.L antikoerper, Epx antikoerper

Hintergrund: Eosinophil peroxidase heavy chain, EPER, EPO, EPP, EPX, EPX PEN, PERE,Eosinophil Peroxidase (EPX)
Cellular localisation: Cytoplasmic, granular

Molekulargewicht: 70kDa (55kDa-Heavy Chain, 15kDa-Light Chain)

Gen-ID: 8288, 279259

UniProt: P11678