Rekombinanter Histone H3.3 Antikörper

Produktnummer ABIN7127092

Produktdetails anti-Histone H3.3 Antikörper

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone H3.3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Phosphohistone H3 (PHH3) is a marker specific for cells undergoing mitosis. Serine 10 of Histone H3 is phosphorylated in association with mitotic chromatin condensation in late G2 and M phase of the cell cycle and thus, PHH3 can distinguish mitosis from apoptotic nuclei. The range of percentage PHH3 positive tumor nuclei was from 0.0 to 6.6% (median value 0.8%). Increased expression of PHH3was significantly associated with tumor thickness (p = 0.031), presence of tumor ulceration (p =0.041) and tumor necrosis (p =0.027), but not with Clark's level of invasion. High levels of PHH3 was associated with increased mitotic count (p = 0.003) and high Ki-67 expression (p = 0.002). For central nervous system tumors, melanoma, soft tissue tumors, GIST, etc., PHH3 mAb is helpful for tumor pathological classification and prognosis.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A synthetic peptide corresponding to (ARK-pS-TGGKAPRKQLc) of Phosphohistone H3 (phospho S10)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil, liver or breast carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Histone H3.3

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Andere Bezeichnung: H3F3 (H3F3A Produkte)

Synonyme: H3.3A antikoerper, H3F3 antikoerper, h3f3a antikoerper, MGC52829 antikoerper, wu:fb58e10 antikoerper, zgc:56193 antikoerper, zgc:86731 antikoerper, H3-I antikoerper, H3-II antikoerper, H3-III antikoerper, H3-IV antikoerper, H3-V antikoerper, H3-VI antikoerper, H3-VII antikoerper, H3-VIII antikoerper, H3F3A antikoerper, h3.3 antikoerper, h3.3a antikoerper, CG5825 antikoerper, Dmel\\CG5825 antikoerper, H3.3 antikoerper, His-3.3A antikoerper, His3.3 antikoerper, PH3 antikoerper, dH3.3A antikoerper, H3 histone family member 3A antikoerper, H3 histone, family 3A antikoerper, H3 histone, family 3A L homeolog antikoerper, H3 histone, family 3C antikoerper, histone H3.3 antikoerper, histone cluster 1, H3a antikoerper, Histone H3.3A antikoerper, H3F3A antikoerper, h3f3a.L antikoerper, h3f3a antikoerper, H3F3C antikoerper, LOC475916 antikoerper, LOC480110 antikoerper, HIST1H3A antikoerper, LOAG_05159 antikoerper, His3.3A antikoerper, H3f3a antikoerper

Hintergrund: Hist1h3a, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, Histone cluster 1, H3a, Histone H3 3 pseudogene, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l,Histone H3, phosphorylated (pSer10) (Nuclear Marker)
Cellular localisation: Nucleus. Chromosome.

Molekulargewicht: 15kDa

Gen-ID: 8350, 132854

UniProt: P68431