Rekombinanter CD5 Antikörper (Extracellular Domain)

Produktnummer ABIN7127141

Produktdetails anti-CD5 Antikörper

Target: CD5

Antikörpertyp: Recombinant Antibody

Bindungsspezifität: Extracellular Domain

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD5 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: The CD5 antigen is a transmembrane glycoprotein expressed on the surface of practically all mature human T-cells (about 10 % of CD4+ T-cells being CD5 negative). In immature (CD34+) T-cells, CD5 is weakly expressed, the intensity of expression increasing with maturation. CD5 is also expressed in a small subset of normal human B-cells (20 % of B-cells in the peripheral blood, scattered cells in the lymph node mantle zone). The CD5+ cells are probably involved in B-T interaction and their ligand is CD72 which is expressed on all B cells. It appears that CD5+ B-cells on activation primarily produce IgM. They also produce more autoantibodies than normal CD5 negative B-cells. Thus, the CD5+ B-cell population is expanded in rheumatoid arthritis and systemic lupus erythematosus. The CD5 antibody reacts with CD5 expressed on B and T cells, and may be a useful aid for the classification of B and T-cell malignancies. This includes B-cell chronic lymphoid leukemia (B-CLL), B-cell small lymphocytic lymphoma (B-SLL), mantle cell lymphoma (MCL) and T-cell lymphoma and leukemia.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length protein corresponding to human CD5 (extracellular). The immunogen contains the specific extracellular domain of huCD5 (D32-P372).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: 293T, Ramos, or MOLT-4 cells. Human tonsil or lymph node.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CD5

Target: CD5

Andere Bezeichnung: CD5 (CD5 Produkte)

Synonyme: CD5 antikoerper, Ly-1 antikoerper, Ly-12 antikoerper, Ly-A antikoerper, Lyt-1 antikoerper, LEU1 antikoerper, T1 antikoerper, CD5 molecule antikoerper, CD5 antigen antikoerper, CD5 molecule like antikoerper, Cd5 molecule antikoerper, CD5 antikoerper, Cd5 antikoerper, CD5L antikoerper

Hintergrund: CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, T-cell surface glycoprotein CD5,CD5 (Mantle Cell Lymphoma Marker)
Cellular localisation: Cell Surface

Molekulargewicht: 67kDa

Gen-ID: 921, 58685

UniProt: P06127