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beta Actin Antikörper (AA 1-50)

Name: beta Actin Antikörper (AA 1-50)
Brand: antibodies-online
SKU: ABIN7127368
Price: 220 EUR
Availability: InStock

ACTB Reaktivität: Human WB, ELISA, IHC, IF, IP, FACS Wirt: Maus Monoclonal 1D12E12B5 unconjugated

Western Blot Positive WB detected in: Rabbit liver tissue, Rabbit brain tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit stomach tissue, A549 whole cell lysate All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin Antikörper (AA 1-50))

IHC image of ABIN7127368 diluted at 1:500 and staining in paraffin-embedded breast cancer performed on a Leica BondTM system. (beta Actin Antikörper (AA 1-50))

IHC

Western Blot Positive WB detected in: Mouse liver tissue, Mouse brain tissue, Mouse lung tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse stomach tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin Antikörper (AA 1-50))

Western Blot Positive WB detected in: Rat liver tissue, Rat brain tissue, Rat lung tissue, Rat stomach tissue, U87 whole cell lysate All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin Antikörper (AA 1-50))

Western Blot Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Mouse kidney tissue, Rabbit kidney tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin Antikörper (AA 1-50))

Western Blot Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, Rat spleen tissue, Rabbit kidney tissue All lanes: ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin Antikörper (AA 1-50))

Western Blot Positive WB detected in: 293T whole cell lysate at 40 μg, 20 μg, 10 μg All lanes:ACTB antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin Antikörper (AA 1-50))

Western Blot Positive WB detected in: 20 μg 293T whole cell lysate ACTB antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 42 KDa Observed band size: 42 KDa Exposure time: 5 min (beta Actin Antikörper (AA 1-50))

IHC image of ABIN7127368 diluted at 1:500 and staining in paraffin-embedded colon cancer performed on a Leica BondTM system. (beta Actin Antikörper (AA 1-50))

IHC

9 Abbildungen

Produktnummer ABIN7127368

Datenblatt (PDF)

Target Alle beta Actin (ACTB) Antikörper anzeigen

beta Actin (ACTB) (Actin, beta (ACTB))
Bindungsspezifität
39

15

15

6

6

5

5

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-50
Reaktivität
176

127

108

56

43

42

37

25

24

22

18

17

12

10

9

7

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1
Human
Wirt
113

78

5

1

1
Maus
Passende Sekundärantikörper
Klonalität
111

85
Monoklonal
Konjugat
124

12

12

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser beta Actin Antikörper ist unkonjugiert
Applikation
164

69

50

45

35

31

25

21

13

13

8

6

6

5

5

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Kreuzreaktivität

Human, Maus, Kaninchen, Ratte

Aufreinigung

Protein A purified

Immunogen

A synthesized peptide derived from human Beta-Actin (1-50AA)

Klon

1D12E12B5

Isotyp

IgG2b

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ACTB Reaktivität: Maus, Ratte, Huhn, Zebrafisch (Danio rerio) WB, IHC, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reaktivität: Human, Maus, Ratte WB, IF, IHC (p), FACS, DNA Mic Wirt: Maus Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB Reaktivität: Human, Maus, Ratte, Schwein, Rind (Kuh) WB, IHC (p) Wirt: Maus Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reaktivität: Human WB, ELISA, IHC (p) Wirt: Maus Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB Reaktivität: Human WB, IF, IHC (p), ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reaktivität: Human, Maus, Huhn, Kaninchen, Schwein, Rind (Kuh), Hamster, Ziege, Fisch WB, ELISA Wirt: Maus Monoclonal 2D1D10 unconjugated

Applikationshinweise

Recommended dilution: WB: 1:5000-1:80000, IHC: 1:500-1:1000, IF: 1:50-1:200, FC: 1:100-1:300, IP: 1 µL-5 µL,

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C,-80 °C

Informationen zur Lagerung

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

beta Actin (ACTB) (Actin, beta (ACTB))

Andere Bezeichnung

ACTB (ACTB Produkte)

Hintergrund

ACTB,ACTB,ACTB,PS1TP5BP1,beta-ac6n,β-ac6n

UniProt

P60709

Pathways

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction