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MCAM Antikörper (AA 50-646)

Name: MCAM Antikörper (AA 50-646)
Brand: antibodies-online
SKU: ABIN7147325
Price: 385 EUR
Availability: InStock

MCAM Reaktivität: Human FACS, WB, IF, ELISA Wirt: Maus Monoclonal 4D10C7 unconjugated

Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: CD146 antibody at 1:1250 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 72, 58 kDa Observed band size: 120 kDa (MCAM Antikörper (AA 50-646))

Overlay histogram showing MCF-7 cells stained with ABIN7147325 (red line) at 1:400. (MCAM Antikörper (AA 50-646))

FACS

Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate All lanes: CD146 antibody at 1:500 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 72, 58 kDa Observed band size: 120 kDa (MCAM Antikörper (AA 50-646))

Immunofluorescence staining of A375 cells with ABIN7147325 at 1:100, counter-stained with DAPI. (MCAM Antikörper (AA 50-646))

Immunofluorescence staining of Hela cells with ABIN7147325 at 1:100, counter-stained with DAPI. (MCAM Antikörper (AA 50-646))

Immunofluorescence staining of MCF-7 cells with ABIN7147325 at 1:100, counter-stained with DAPI. (MCAM Antikörper (AA 50-646))

Immunofluorescence staining of THP-1 cells with ABIN7147325 at 1:100, counter-stained with DAPI. (MCAM Antikörper (AA 50-646))

7 Abbildungen

Produktnummer ABIN7147325

Datenblatt (PDF)

Target Alle MCAM Antikörper anzeigen

MCAM (Melanoma Cell Adhesion Molecule (MCAM))
Bindungsspezifität
16

13

11

5

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 50-646
Reaktivität
133

53

47

14

14

9

8

7

6

2
Human
Wirt
95

62

8

1

1
Maus
Passende Sekundärantikörper
Klonalität
88

79
Monoklonal
Konjugat
70

14

14

11

6

5

4

4

4

3

3

3

3

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser MCAM Antikörper ist unkonjugiert
Applikation
85

79

69

42

21

20

14

10

10

5

4

4

3

3

2

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), ELISA

Kreuzreaktivität

Human

Aufreinigung

Protein G purified

Immunogen

Recombinant Human Cell surface glycoprotein MUC18 protein (50-646AA)

Klon

4D10C7

Isotyp

IgG2a

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Applikationshinweise

Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200,

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C,-80 °C

Informationen zur Lagerung

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Andere Bezeichnung

CD146 (MCAM Produkte)

Synonyme

CD146 antikoerper, MUC18 antikoerper, Muc18 antikoerper, 1-gicerin antikoerper, AV025631 antikoerper, CD149 antikoerper, s-endo antikoerper, s-gicerin antikoerper, cd146 antikoerper, gicerin antikoerper, id:ibd3512 antikoerper, mcam antikoerper, MCAM antikoerper, melanoma cell adhesion molecule antikoerper, melanoma cell adhesion molecule b antikoerper, MCAM antikoerper, Mcam antikoerper, mcamb antikoerper

Hintergrund

Background: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Aliases: Cell surface glycoprotein MUC18 (Cell surface glycoprotein P1H12) (Melanoma cell adhesion molecule) (Melanoma-associated antigen A32) (Melanoma-associated antigen MUC18) (S-endo 1 endothelial-associated antigen) (CD antigen CD146), MCAM, MUC18

UniProt

P43121