MAPKAP Kinase 5 Antikörper (pThr182)
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- Target Alle MAPKAP Kinase 5 (MAPKAPK5) Antikörper anzeigen
- MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
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Bindungsspezifität
- pThr182
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Reaktivität
- Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser MAPKAP Kinase 5 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Spezifität
- This phosphorylation site is homologous to that of Thr182 in Mouse.
- Kreuzreaktivität
- Ratte
- Homologie
- Human,Mouse,Dog,Cow,Pig,Horse,Chicken,Rabbit
- Aufreinigung
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic phosphopeptide derived from human MAPKAPK5 around the phosphorylation site of Thr182
- Isotyp
- IgG
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- Applikationshinweise
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WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Konservierungsmittel
- ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
- Andere Bezeichnung
- MAPKAPK5 (MAPKAPK5 Produkte)
- Synonyme
- MAPKAPK5 antikoerper, MK5 antikoerper, PRAK antikoerper, MAPKAP-K5 antikoerper, MK-5 antikoerper, mitogen-activated protein kinase-activated protein kinase 5 antikoerper, MAP kinase-activated protein kinase 5 antikoerper, MAPKAPK5 antikoerper, mapkapk5 antikoerper, Mapkapk5 antikoerper
- Hintergrund
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Synonyms: MK5, MK-5, PRAK, MAPKAP-K5, MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAPK-5, p38-regulated/activated protein kinase, MAPKAPK5
Background: Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34C, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.
- Gen-ID
- 8550
- UniProt
- Q8IW41
- Pathways
- MAPK Signalweg
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