CLOCK Antikörper (AA 577-846)

Produktnummer ABIN7147792

Produktdetails anti-CLOCK Antikörper

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Bindungsspezifität: AA 577-846

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CLOCK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Kreuzreaktivität: Human

Aufreinigung: Antigen Affinity Purified

Immunogen: Recombinant Human Circadian locomoter output cycles protein kaput protein (577-846AA)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu CLOCK

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Andere Bezeichnung: CLOCK (CLOCK Produkte)

Synonyme: CG7391 antikoerper, CLK antikoerper, CLOCK antikoerper, Dmel\\CG7391 antikoerper, Jerk antikoerper, Jrk antikoerper, PAS1 antikoerper, bHLHe10 antikoerper, clk antikoerper, clock antikoerper, dCLK antikoerper, dCLK/JRK antikoerper, dCLOCK antikoerper, dClck antikoerper, dClk antikoerper, dClock antikoerper, dclk antikoerper, jrk antikoerper, Xclk antikoerper, XClock antikoerper, zCLOCK1 antikoerper, zfCLOCK1 antikoerper, 5330400M04Rik antikoerper, KAT13D antikoerper, bHLHe8 antikoerper, mKIAA0334 antikoerper, Clock antikoerper, clock circadian regulator antikoerper, clock homolog (mouse) antikoerper, clock circadian regulator a antikoerper, circadian locomotor output cycles kaput antikoerper, clock circadian regulator L homeolog antikoerper, Clk antikoerper, CLOCK antikoerper, clock antikoerper, clocka antikoerper, Clock antikoerper, clock.L antikoerper

Hintergrund:

Background: Transcriptional activator which forms a core component of the circadian clock. The circadian clock, an internal time-keeping system, regulates various physiological processes through the generation of approximately 24 hour circadian rhythms in gene expression, which are translated into rhythms in metabolism and behavior. It is derived from the Latin roots \\\'circa\\\' (about) and \\\'diem\\\' (day) and acts as an important regulator of a wide array of physiological functions including metabolism, sleep, body temperature, blood pressure, endocrine, immune, cardiovascular, and renal function. Consists of two major components: the central clock, residing in the suprachiasmatic nucleus (SCN) of the brain, and the peripheral clocks that are present in nearly every tissue and organ system. Both the central and peripheral clocks can be reset by environmental cues, also known as Zeitgebers (German for \\\'timegivers\\\'). The predominant Zeitgeber for the central clock is light, which is sensed by retina and signals directly to the SCN. The central clock entrains the peripheral clocks through neuronal and hormonal signals, body temperature and feeding-related cues, aligning all clocks with the external light/dark cycle. Circadian rhythms allow an organism to achieve temporal homeostasis with its environment at the molecular level by regulating gene expression to create a peak of protein expression once every 24 hours to control when a particular physiological process is most active with respect to the solar day. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) plays a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms (tau refers to the period of a rhythm and is the length, in time, of one complete cycle). A diurnal rhythm is synchronized with the day/night cycle, while the ultradian and infradian rhythms have a period shorter and longer than 24 hours, respectively. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndromes and aging. A transcription/translation feedback loop (TTFL) forms the core of the molecular circadian clock mechanism. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop, act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes (involved in key metabolic processes), harboring E-box elements (5\\\'-CACGTG-3\\\') within their promoters. The core clock genes: PER1/2/3 and CRY1/2 which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. This heterodimer also activates nuclear receptors NR1D1/2 and RORA/B/G, which form a second feedback loop and which activate and repress ARNTL/BMAL1 transcription, respectively. CLOCK has an intrinsic acetyltransferase activity, which enables circadian chromatin remodeling by acetylating histones and nonhistone proteins, including its own partner ARNTL/BMAL1. Regulates the circadian expression of ICAM1, VCAM1, CCL2, THPO and MPL and also acts as an enhancer of the transactivation potential of NF-kappaB. Plays an important role in the homeostatic regulation of sleep. The CLOCK-ARNTL/BMAL1 heterodimer regulates the circadian expression of SERPINE1/PAI1, VWF, B3, CCRN4L/NOC, NAMPT, DBP, MYOD1, PPARGC1A, PPARGC1B, SIRT1, GYS2, F7, NGFR, GNRHR, BHLHE40/DEC1, ATF4, MTA1, KLF10 and also genes implicated in glucose and lipid metabolism. Represses glucocorticoid receptor NR3C1/GR-induced transcriptional activity by reducing the association of NR3C1/GR to glucocorticoid response elements (GREs) via the acetylation of multiple lysine residues located in its hinge region. Promotes rhythmic chromatin opening, regulating the DNA accessibility of other transcription factors. The CLOCK-ARNTL2/BMAL2 heterodimer activates the transcription of SERPINE1/PAI1 and BHLHE40/DEC1.

Aliases: bHLHe8 antibody, Circadian locomoter output cycles kaput protein antibody, Circadian locomoter output cycles protein kaput antibody, Circadian Locomotor Output Cycles Kaput antibody, Circadium Locomotor Output Cycles Kaput antibody, Class E basic helix-loop-helix protein 8 antibody, CLOCK antibody, Clock circadian regulator antibody, Clock homolog antibody, Clock protein antibody, CLOCK_HUMAN antibody, hCLOCK antibody, KIAA0334 antibody

UniProt: O15516

Pathways: Regulation of Lipid Metabolism by PPARalpha, Photoperiodism