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Hsc70 Antikörper (AA 2-646)

Name: Hsc70 Antikörper (AA 2-646)
Brand: antibodies-online
SKU: ABIN7154883
Price: 385 EUR
Availability: InStock

HSPA8 Reaktivität: Human WB, IHC, ELISA, IP, FACS Wirt: Maus Monoclonal 2G8F6 unconjugated

Western Blot Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Mouse spleen tissue, Rat spleen tissue, Mouse heart tissue, Rat heart tissue All lanes HSPA8 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 10s (Hsc70 Antikörper (AA 2-646))

Overlay histogram showing MCF-7 cells stained with ABIN7154883 (red line). (Hsc70 Antikörper (AA 2-646))

FACS

Western Blot Positive WB detected in: Hela whole cell lysate at 20 μg, 10 μg, 5 μg, 2. (Hsc70 Antikörper (AA 2-646))

Western Blot Positive WB detected in: 20 μg Hela whole cell lysate HSPA8 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 10s (Hsc70 Antikörper (AA 2-646))

IHC image of ABIN7154883 diluted at 1:256 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. (Hsc70 Antikörper (AA 2-646))

IHC

Immunoprecipitating HSPA8 in Hela whole cell lysate Lane 1: Mouse control IgG2b instead of ABIN7154883 in Hela whole cell lysate. (Hsc70 Antikörper (AA 2-646))

7 Abbildungen

Produktnummer ABIN7154883

Datenblatt (PDF)

Target Alle Hsc70 (HSPA8) Antikörper anzeigen

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
Bindungsspezifität
14

7

6

4

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 2-646
Reaktivität
83

48

44

21

17

14

13

11

11

11

10

10

5

5

5

4

4

3

3

3

2

1

1

1

1

1

1

1
Human
Wirt
72

27

7

2
Maus
Passende Sekundärantikörper
Klonalität
69

38
Monoklonal
Konjugat
60

19

11

3

3

1

1

1

1

1

1

1

1

1

1

1

1
Dieser Hsc70 Antikörper ist unkonjugiert
Applikation
94

50

40

30

29

28

16

13

11

11

11

5

4

2

2

2

2
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Kreuzreaktivität

Human, Maus, Ratte

Aufreinigung

Protein A purified

Immunogen

Recombinant Human Heat shock cognate 71 kDa protein (2-646AA)

Klon

2G8F6

Isotyp

IgG2b

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anti-Heat Shock 70kDa Protein 8 (HSPA8) antibody
HSPA8 Reaktivität: Human WB, IHC, ELISA, IP, ICC, IF, Bind, PLA, AA Wirt: Maus Monoclonal 1F2-H5 unconjugated

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HSPA8 Reaktivität: Human, Ratte, Maus WB, ICC, IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (N-Term) antibody
HSPA8 Reaktivität: Human, Ratte, Maus, Rind (Kuh), Schaf, Schwein, Zebrafisch (Danio rerio), Ziege WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 534-615) antibody
HSPA8 Reaktivität: Maus WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 418-597) antibody
HSPA8 Reaktivität: Human WB, IHC, ELISA Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

Recommended dilution: WB: 1:10000-1:256000, IHC: 1:100-1:500, FC: 1:100-1:300, IP: 1 μL-4 μL,

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C,-80 °C

Informationen zur Lagerung

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Andere Bezeichnung

HSPA8 (HSPA8 Produkte)

Hintergrund

Background: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP
Aliases: Heat shock cognate 71 kDa protein (Heat shock 70 kDa protein 8) (Lipopolysaccharide-associated protein 1) (LAP-1) (LPS-associated protein 1), HSPA8, HSC70 HSP73 HSPA10

UniProt

P11142