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Hsc70 Antikörper (AA 2-646)

Name: Hsc70 Antikörper (AA 2-646)
Brand: antibodies-online
SKU: ABIN7154883
Price: 385 EUR
Availability: InStock

HSPA8 Reaktivität: Human WB, IHC, ELISA, IP, FACS Wirt: Maus Monoclonal 2G8F6 unconjugated

Western Blot Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Mouse spleen tissue, Rat spleen tissue, Mouse heart tissue, Rat heart tissue All lanes HSPA8 antibody at 1:2000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 10s (Hsc70 Antikörper (AA 2-646))

Overlay histogram showing MCF-7 cells stained with ABIN7154883 (red line). (Hsc70 Antikörper (AA 2-646))

FACS

Western Blot Positive WB detected in: Hela whole cell lysate at 20 μg, 10 μg, 5 μg, 2. (Hsc70 Antikörper (AA 2-646))

Western Blot Positive WB detected in: 20 μg Hela whole cell lysate HSPA8 antibody at 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 70~75 KDa Observed band size: 70~75 KDa Exposure time: 10s (Hsc70 Antikörper (AA 2-646))

IHC image of ABIN7154883 diluted at 1:256 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. (Hsc70 Antikörper (AA 2-646))

IHC

Immunoprecipitating HSPA8 in Hela whole cell lysate Lane 1: Mouse control IgG2b instead of ABIN7154883 in Hela whole cell lysate. (Hsc70 Antikörper (AA 2-646))

7 Abbildungen

Produktnummer ABIN7154883

Datenblatt (PDF)

Target Alle Hsc70 (HSPA8) Antikörper anzeigen

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
Bindungsspezifität
14

7

6

5

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 2-646
Reaktivität
81

48

44

21

17

14

13

11

11

11

10

10

5

5

5

4

4

3

3

3

2

1

1

1

1

1

1

1
Human
Wirt
70

27

7

2
Maus
Passende Sekundärantikörper
Klonalität
70

35
Monoklonal
Konjugat
58

19

11

3

3

1

1

1

1

1

1

1

1

1

1

1

1
Dieser Hsc70 Antikörper ist unkonjugiert
Applikation
92

51

39

29

28

28

15

13

11

11

11

5

4

2

2

2

2

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Kreuzreaktivität

Human, Maus, Ratte

Aufreinigung

Protein A purified

Immunogen

Recombinant Human Heat shock cognate 71 kDa protein (2-646AA)

Klon

2G8F6

Isotyp

IgG2b

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anti-Heat Shock 70kDa Protein 8 (HSPA8) (AA 534-615) antibody
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HSPA8 Reaktivität: Human WB, IHC, ELISA Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

Recommended dilution: WB: 1:10000-1:256000, IHC: 1:100-1:500, FC: 1:100-1:300, IP: 1 μL-4 μL,

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C,-80 °C

Informationen zur Lagerung

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Andere Bezeichnung

HSPA8 (HSPA8 Produkte)

Synonyme

hsc54 antikoerper, hsc70 antikoerper, hsc71 antikoerper, hsp71 antikoerper, hsp73 antikoerper, hspa10 antikoerper, lap1 antikoerper, nip71 antikoerper, HSC54 antikoerper, HSC70 antikoerper, HSC71 antikoerper, HSP71 antikoerper, HSP73 antikoerper, HSPA10 antikoerper, LAP1 antikoerper, NIP71 antikoerper, Hsc70 antikoerper, 2410008N15Rik antikoerper, Hsc71 antikoerper, Hsc73 antikoerper, Hsp73 antikoerper, Hspa10 antikoerper, wu:fb01g06 antikoerper, wu:fi48b06 antikoerper, heat shock protein family A (Hsp70) member 8 L homeolog antikoerper, heat shock protein family A (Hsp70) member 8 antikoerper, heat shock 70kDa protein 8 antikoerper, heat shock protein 8 antikoerper, hspa8.L antikoerper, HSPA8 antikoerper, Hspa8 antikoerper, hspa8 antikoerper

Hintergrund

Background: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP
Aliases: Heat shock cognate 71 kDa protein (Heat shock 70 kDa protein 8) (Lipopolysaccharide-associated protein 1) (LAP-1) (LPS-associated protein 1), HSPA8, HSC70 HSP73 HSPA10

UniProt

P11142