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CD14 Antikörper (AA 20-345)

Name: CD14 Antikörper (AA 20-345)
Brand: antibodies-online
SKU: ABIN7160185
Price: 385 EUR
Availability: InStock

CD14 Reaktivität: Human FACS, WB, IHC, ELISA, IF Wirt: Maus Monoclonal 14G1F3 unconjugated

Western Blot Positive WB detected in: Rabbit spleen tissue, Rabbit small intestine tissue All lanes: CD14 antibody at 1:2500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 41 kDa Observed band size: 55 kDa (CD14 Antikörper (AA 20-345))

Immunofluorescence staining of A549 cells with ABIN7160185 at 1:90, counter-stained with DAPI. (CD14 Antikörper (AA 20-345))

Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate All lanes: CD14 antibody at 1:1800 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 41 kDa Observed band size: 55 kDa (CD14 Antikörper (AA 20-345))

IHC image of ABIN7160185 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. (CD14 Antikörper (AA 20-345))

9 Abbildungen

Produktnummer ABIN7160185

Datenblatt (PDF)

Target Alle CD14 Antikörper anzeigen

CD14
Bindungsspezifität
9

5

5

5

4

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 20-345
Reaktivität
244

46

32

25

24

18

17

16

15

14

13

10

9

9

8

8

8

5

2

2

2

1

1

1
Human
Wirt
213

52

25

3

1
Maus
Passende Sekundärantikörper
Klonalität
240

54
Monoklonal
Konjugat
115

31

24

18

15

8

6

5

5

5

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser CD14 Antikörper ist unkonjugiert
Applikation
219

83

58

56

52

48

21

14

13

12

7

6

4

4

3

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Kreuzreaktivität

Human, Maus, Kaninchen

Aufreinigung

Protein A purified

Immunogen

Recombinant Human Monocyte differentiation antigen CD14 protein (20-345AA)

Klon

14G1F3

Isotyp

IgG1, IgG1 kappa

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Applikationshinweise

Recommended dilution: WB:1:1000-1:5000, IHC:1:50-1:200, IF:1:50-1:200,

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C,-80 °C

Informationen zur Lagerung

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

CD14

Andere Bezeichnung

CD14 (CD14 Produkte)

Synonyme

CD14 molecule antikoerper, CD14 antigen antikoerper, CD14 antikoerper, Cd14 antikoerper

Hintergrund

Background: Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (PubMed:23880187).
Aliases: Monocyte differentiation antigen CD14 (Myeloid cell-specific leucine-rich glycoprotein) (CD antigen CD14) [Cleaved into: Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form], CD14

UniProt

P08571

Pathways

TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades