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PKM Antikörper (AA 2-531)

Name: PKM Antikörper (AA 2-531)
Brand: antibodies-online
SKU: ABIN7166916
Price: 423.5 EUR
Availability: InStock

PKM Reaktivität: Human ELISA, WB, IHC, IF, IP, FACS Wirt: Maus Monoclonal 6C3C7 unconjugated

Western Blot Positive WB detected in: A549 whole cell lysate, 293T whole cell lysate, U251 whole cell lysate, Rat Brain tissue, Rabbit Skeletal Muscle tissue All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 1 min (PKM Antikörper (AA 2-531))

IHC image of ABIN7166916 diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. (PKM Antikörper (AA 2-531))

IHC

Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 1 min (PKM Antikörper (AA 2-531))

Western Blot Positive WB detected in: Mouse Heart tissue, Mouse Brain tissue, Mouse Skeletal Muscle tissue All lanes: PKM antibody at 1:4000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 55-60 kDa Observed band size: 55-60 kDa (PKM Antikörper (AA 2-531))

Western Blot Positive WB detected in: Rat Heart tissue, Rat Spleen tissue, Rat Brain tissue All lanes: PKM antibody at 1:4000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 55-60 kDa Observed band size: 55-60 kDa (PKM Antikörper (AA 2-531))

Western Blot Positive WB detected in: MCF-7 whole cell lysate at 40 μg, 20 μg, 10 μg, 5 μg, 2. (PKM Antikörper (AA 2-531))

Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: PKM antibody at 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 5 min (PKM Antikörper (AA 2-531))

IHC

9 Abbildungen

Produktnummer ABIN7166916

Datenblatt (PDF)

Target Alle PKM Antikörper anzeigen

PKM (Pyruvate Kinase M1/2 (PKM))
Bindungsspezifität
5

5

4

2

2

2

1

1

1

1

1

1

1
AA 2-531
Reaktivität
37

11

5
Human
Wirt
35

4
Maus
Passende Sekundärantikörper
Klonalität
34

5
Monoklonal
Konjugat
22

4

4

3

1

1

1

1

1

1
Dieser PKM Antikörper ist unkonjugiert
Applikation
24

18

11

8

4

3

2

2

1

1
ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Kreuzreaktivität

Human, Maus, Kaninchen, Ratte

Aufreinigung

Protein G purified

Immunogen

Recombinant Human Pyruvate kinase PKM protein (2-531AA)

Klon

6C3C7

Isotyp

IgG1, IgG1 kappa
anti-Pyruvate Kinase M1/2 (PKM) (AA 11-221) antibody
PKM Reaktivität: Human WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) antibody
PKM Reaktivität: Human ELISA, WB, IHC, IF, IP, FACS Wirt: Kaninchen Monoclonal 7B2 unconjugated Recombinant Antibody

anti-Pyruvate Kinase M1/2 (PKM) (AA 185-461) antibody
PKM Reaktivität: Human ELISA, WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (Internal Region) antibody
PKM Reaktivität: Human, Maus, Ratte ELISA, WB, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (AA 2-192) antibody
PKM Reaktivität: Human ELISA, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (pSer37) antibody
PKM Reaktivität: Human ELISA, WB, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) antibody
PKM Reaktivität: Human, Maus, Ratte ELISA, WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (pSer37) antibody
PKM Reaktivität: Human, Maus, Ratte ELISA, WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (pTyr105) antibody
PKM Reaktivität: Human, Maus ELISA, WB, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

anti-Pyruvate Kinase M1/2 (PKM) (AA 475-500), (N-Term) antibody
PKM Reaktivität: Human WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

Recommended dilution: WB: 1:4000-1:256000, IHC: 1:200-1:500, IF: 1:150-1:300, FC: 1:50-1:200, IP: 1 μL-4 μL,

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C,-80 °C

Informationen zur Lagerung

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

PKM (Pyruvate Kinase M1/2 (PKM))

Andere Bezeichnung

PKM (PKM Produkte)

Hintergrund

Background: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Aliases: CTHBP antibody, Cytosolic thyroid hormone-binding protein antibody, KPYM_HUMAN antibody, OIP-3 antibody, Opa-interacting protein 3 antibody, p58 antibody, pkm antibody, PKM1 antibody, PKM2 antibody, Pyruvate kinase 2/3 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase PKM antibody, THBP1 antibody, Thyroid hormone-binding protein 1 antibody, Tumor M2-PK antibody

UniProt

P14618

Pathways

Warburg Effekt