GBL Antikörper (AA 159-220)

Produktnummer ABIN7171522

Produktdetails anti-GBL Antikörper

Target: GBL (G protein beta subunit-like (GBL))

Bindungsspezifität: AA 159-220

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GBL Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Kreuzreaktivität: Human

Aufreinigung: >95%, Protein G purified

Immunogen: Recombinant Human Target of rapamycin complex subunit LST8 protein (159-220AA)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu GBL

Target: GBL (G protein beta subunit-like (GBL))

Andere Bezeichnung: MLST8 (GBL Produkte)

Synonyme: gbl antikoerper, lts8 antikoerper, GBL antikoerper, GbetaL antikoerper, LST8 antikoerper, POP3 antikoerper, WAT1 antikoerper, 0610033N12Rik antikoerper, AA409454 antikoerper, AI505104 antikoerper, AI851821 antikoerper, Gbl antikoerper, fi37e04 antikoerper, wu:fi37e04 antikoerper, zgc:55455 antikoerper, zgc:85668 antikoerper, MTOR associated protein, LST8 homolog L homeolog antikoerper, MTOR associated protein, LST8 homolog antikoerper, MTOR associated protein, LST8 homolog (S. cerevisiae) antikoerper, mlst8.L antikoerper, MLST8 antikoerper, Mlst8 antikoerper, mlst8 antikoerper

Hintergrund:

Background: Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at \'Thr-389\', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 \'Ser-473\' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on \'Thr-308\' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at \'Ser-422\'. mTORC2 also modulates the phosphorylation of PRKCA on \'Ser-657\'.

Aliases: G protein beta subunit like antibody, G protein beta subunit-like antibody, Gable antibody, GbetaL antibody, GBL antibody, GBL protein antibody, LST8 antibody, LST8_HUMAN antibody, Mammalian lethal with SEC13 protein 8 antibody, MGC111011 antibody, mLST8 antibody, MTOR associated protein LST8 homolog (S. cerevisiae) antibody, POP3 antibody, POP3 homolog (S. pombe) antibody, Protein GbetaL antibody, Target of rapamycin complex subunit LST8 antibody, TORC subunit LST8 antibody, WAT1 antibody, WAT1 homolog (S. pombe) antibody

UniProt: Q9BVC4

Pathways: PI3K-Akt Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Autophagie, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effekt