RAG2 Antikörper (AA 296-415) (Biotin)

Produktnummer ABIN7175271

Produktdetails anti-RAG2 Antikörper (Biotin)

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Bindungsspezifität: AA 296-415

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAG2 Antikörper ist konjugiert mit Biotin

Applikation: ELISA

Kreuzreaktivität: Human

Aufreinigung: >95%, Protein G purified

Immunogen: Recombinant Human V(D)J recombination-activating protein 2 protein (296-415AA)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu RAG2

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Andere Bezeichnung: RAG2 (RAG2 Produkte)

Synonyme: RAG-2 antikoerper, Rag-2 antikoerper, rag-2 antikoerper, zgc:136743 antikoerper, recombination activating 2 antikoerper, recombination activating gene 2 antikoerper, recombination activating gene 2 S homeolog antikoerper, RAG2 antikoerper, Rag2 antikoerper, rag2 antikoerper, rag2.S antikoerper

Hintergrund:

Background: Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3\'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5\'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at \'Lys-4\' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3 (By similarity).

Aliases: RAG 2 antibody, RAG-2 antibody, RAG2 antibody, RAG2_HUMAN antibody, Recombination activating gene 2 antibody, V(D)J recombination activating protein 2 antibody, V(D)J recombination-activating protein 2 antibody

UniProt: P55895

Pathways: Chromatin Binding, Production of Molecular Mediator of Immune Response