PAK1 Antikörper (Ser199)

Produktnummer ABIN7180064

Produktdetails anti-PAK1 Antikörper

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Bindungsspezifität: Ser199

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized non-phosphopeptide derived from Human PAK1/2 around the phosphorylation site of serine 199 (T-K-S(p)-V-Y).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB:1:500-1:3000, IHC:1:50-1:100,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Andere Bezeichnung: PAK1 (PAK1 Produkte)

Synonyme: X-PAK1 antikoerper, XPak1 antikoerper, pak antikoerper, pak-1 antikoerper, pakalpha antikoerper, PAKalpha antikoerper, AW045634 antikoerper, PAK-1 antikoerper, Paka antikoerper, p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog antikoerper, p21 (RAC1) activated kinase 1 antikoerper, p21 protein (Cdc42/Rac)-activated kinase 1 antikoerper, Serine/threonine-protein kinase pak-1 antikoerper, pak1.S antikoerper, PAK1 antikoerper, Pak1 antikoerper, pak-1 antikoerper

Hintergrund:

Background: Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at 'Ser-246' promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation.

Brown J.L., Curr. Biol. 6:598-605(1996).
Sells M.A., Curr. Biol. 7:202-210(1997).
Manser E., Mol. Cell. Biol. 17:1129-1143(1997)

Aliases: ADRB2 antibody, Alpha PAK antibody, Alpha-PAK antibody, MGC130000 antibody, MGC130001 antibody, p21 activated kinase 1 antibody, p21 protein (Cdc42/Rac) activated kinase 1 antibody, p21-activated kinase 1 antibody, p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody, p65 PAK antibody, p65-PAK antibody, P68-PAK antibody, PAK alpha antibody, PAK-1 antibody, Pak1 antibody, PAK1_HUMAN antibody, Paka antibody, PAKalpha antibody, Protein kinase MUK2 antibody, Rac/p21-activated kinase antibody, Serine/threonine-protein kinase PAK 1 antibody, STE20 homolog yeast antibody

UniProt: Q13153

Pathways: MAPK Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis