tdTomato Antikörper (DyLight 488)
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- Target Alle tdTomato Produkte
- tdTomato (tdTomato Fluorescent Protein (tdTomato))
- Reaktivität
- Discosoma
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser tdTomato Antikörper ist konjugiert mit DyLight 488
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Applikation
- Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
- Kreuzreaktivität (Details)
- Reacts against tdTomato, mCherry, red fluorescent protein (DsRed) from so-called disc corals of Discosoma species.
- Aufreinigung
- Epitope affinity purified
- Immunogen
- Purified recombinant peptide produced in E. coli
- Isotyp
- IgG
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- Applikationshinweise
- WB:1:500-1:2,000, IF:1:50-1:500, IHC-F:1:50-1:1,000
- Kommentare
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In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 2.5 mg/mL
- Buffer
- PBS, 20 % glycerol and 0.05 % sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store at -20 °C for long-term storage. Store at 2-8 °C for up to one month.
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- Target
- tdTomato (tdTomato Fluorescent Protein (tdTomato))
- Andere Bezeichnung
- tdTomato (tdTomato Produkte)
- Hintergrund
- TdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength, makes it ideal for live animal research.
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