gamma H2AX Antikörper (Ser140)

Produktnummer ABIN7449557

Produktdetails anti-gamma H2AX Antikörper

Target: gamma H2AX (GAMMA-H2AX) (gamma Histone Variant H2AX (GAMMA-H2AX))

Bindungsspezifität: Ser140

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser gamma H2AX Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC)

Verwendungszweck: Rabbit anti-gamma-H2AX Antibody, Affinity Purified

Aufreinigung: Affinity Purified

Immunogen: surrounding serine 140

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ICC-IF: 1:500 - 1:5,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

IHC: 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

WB: 1:10,000 - 1:25,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu gamma H2AX

Target: gamma H2AX (GAMMA-H2AX) (gamma Histone Variant H2AX (GAMMA-H2AX))

Andere Bezeichnung: gamma-H2AX (GAMMA-H2AX Produkte)

Hintergrund: Background: H2AX is a member of the histone H2A family. The four core histones involved in the formation of the nucleosome structure of compacted chromatin are H2A, H2B, H3, and H4. H2AX may function to facilitate DNA repair, and recent studies have shown that H2AX is required for the maintenance of genomic stability. Gamma H2AX is the phosphorylated form of H2AX that results in response to DNA damage.

Gen-ID: 3014

NCBI Accession: NP_002096

UniProt: P16104