RNA Polymerase II Antikörper (AA 754-804)

Produktnummer ABIN7453315

Produktdetails anti-RNA Polymerase II Antikörper

Target: RNA Polymerase II

Bindungsspezifität: AA 754-804

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RNA Polymerase II Antikörper ist unkonjugiert

Applikation: Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Verwendungszweck: Rabbit anti-RNA Polymerase II Antibody, Affinity Purified

Homologie: Mouse

Aufreinigung: Affinity Purified

Immunogen: Between AA 754 and 804

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IP: 2 - 10 μg/mg lysate

WB: Not recommended. ABIN7453315 has not performed satisfactorily when used for WB of RNA Polymerase II in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RNA Polymerase II.

ChIP-PCR: 8 μg per IP

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu RNA Polymerase II

Target: RNA Polymerase II

Abstract: RNA Polymerase II Produkte

Synonyme: DNA-directed RNA polymerase II subunit RPB7 antikoerper, RNA polymerase II antikoerper

Hintergrund: Background: RNA polymerase II is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. RNA polymerase II subunit B1 (RPB1) is the largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. It forms the polymerase active center together with the second largest subunit [taken from NCBI Entrez Gene (Gene ID: 5430)].

Gen-ID: 5430

NCBI Accession: NP_000928

UniProt: P24928