COBRA1 Antikörper (AA 530-580)

Produktnummer ABIN7454796

Produktdetails anti-COBRA1 Antikörper

Target: COBRA1 (Cofactor of BRCA1 (COBRA1))

Bindungsspezifität: AA 530-580

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COBRA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Verwendungszweck: Rabbit anti-COBRA1 Antibody, Affinity Purified

Aufreinigung: Affinity Purified

Immunogen: between AA 530 and 580

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:200 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu COBRA1

Target: COBRA1 (Cofactor of BRCA1 (COBRA1))

Andere Bezeichnung: COBRA1 (COBRA1 Produkte)

Hintergrund: Background: COBRA1 (Cofactor of BRCA1) is also known as NELFB (negative elongation factor E). NELFB is one of four subunits that make up the NELF transcription elongation factor. The four subunits of NELF are NELF A (WHSC2), NELFB, either NELFC or NELFD, and NELFE. NELF, along with DRB sensitivity-inducing factor (DSIF), functions to cause transcriptional pausing of RNA polymerase II. COBRA1 was originally identified in a yeast two-hybrid screen of a human ovary cDNA library using the BRCA COOH terminus repeat, BRCT1, as the bait protein. In this study, COBRA1 was found to be involved in BRCT1-mediated chromatin remodeling. COBRA1 has also been found to interact with several nuclear receptors such as ERalpha and androgen receptor to modulate their transcriptional activity.

Gen-ID: 25920

NCBI Accession: NP_056271

UniProt: Q8WX92