Rekombinanter Elastase 3B Antikörper (AA 82-238)

Produktnummer ABIN7477421

Produktdetails anti-Elastase 3B Antikörper

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Antikörpertyp: Recombinant Antibody

Bindungsspezifität: AA 82-238

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Elastase 3B Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Immunogen: Recombinant human CELA3B protein fragment (around aa 82-238) (exact sequence is proprietary)

Klon: MSVA-410M

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Pancreas: Acinar cells should show a strong CELA3B immunostaining with apical predominance.

Negative Control: Kidney: CELA3B staining should be absent in all epithelial cells.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu Elastase 3B

Target: Elastase 3B (ELA3B) (Elastase 3B, Pancreatic (ELA3B))

Andere Bezeichnung: CELA3B (ELA3B Produkte)

Hintergrund: Chymotrypsin like elastase family member 3B (CELA3B), ELA3B, Elastase IIIB, Protease E,CELA3B antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P08861