Rekombinanter KRT14 Antikörper (AA 350-472)

Produktnummer ABIN7477443

Produktdetails anti-KRT14 Antikörper

Target: KRT14 (Keratin 14 (KRT14))

Antikörpertyp: Recombinant Antibody

Bindungsspezifität: AA 350-472

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser KRT14 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Immunogen: Recombinant human KRT14 fragment (around aa350-472) (exact sequence is proprietary)

Klon: MSVA-614R

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Tonsil: A strong KRT14 immunostaining should be seen in the surface and crypt epithelium.

Negative Control: Tonsil: KRT14 immunostaining should be absent in all non-epithelial cells.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu KRT14

Target: KRT14 (Keratin 14 (KRT14))

Andere Bezeichnung: Cytokeratin 14 (KRT14 Produkte)

Hintergrund: CK-14, Dowling Meara, ebs3, ebs4, Epidermolysis Bullosa Simplex, Keratin Type I Cytoskeletal 14, Koebner, NFJ,Cytokeratin 14 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P02533