Rekombinanter Desmin Antikörper

Produktnummer ABIN7477457

Produktdetails anti-Desmin Antikörper

Target: Desmin (DES)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Immunogen: Recombinant full-length human desmin protein

Klon: MSVA-651M

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Appendix: Smooth muscle cells in the lamina muscularis mucosae and muscularis propria must show a strong cytoplasmic staining while staining should be at least moderate in most smooth muscle cells of blood vessels and in dispersed myofibroblasts.

Negative Control: Appendix: Desmin staining should not be seen in lymphocytes and epithelial cells.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu Desmin

Target: Desmin (DES)

Andere Bezeichnung: Desmin (DES Produkte)

Hintergrund: CMD1I, CSM1, CSM2, DES, Intermediate filament protein,Desmin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P17661