MCM3 Antikörper

Produktnummer ABIN7477513

Produktdetails anti-MCM3 Antikörper

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MCM3 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-503M

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Colon: A strong nuclear MCM3 immunostaining should be seen in virtually all crypt base cells.

Negative Control: Colon: MCM3 immunostaining should be largely absent in surface epithelial cells and in most stroma cells.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu MCM3

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Andere Bezeichnung: MCM3 (MCM3 Produkte)

Hintergrund: Minichromosome Maintenance Complex Component 3, DNA Polymerase Alpha Holoenzyme-Associated Protein P1, DNA Replication Licensing Factor MCM3, RLF Subunit Beta, P1-MCM3, P102,MCM3 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P25205

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA