Rekombinanter MCM7 Antikörper (AA 195-319)

Produktnummer ABIN7477515

Produktdetails anti-MCM7 Antikörper

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Antikörpertyp: Recombinant Antibody

Bindungsspezifität: AA 195-319

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser MCM7 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Immunogen: Recombinant human MCM7 protein fragment (aa195-319)

Klon: MSVA-507R

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Colon: A strong nuclear MCM7 immunostaining should be seen in virtually all crypt base cells.

Negative Control: Colon: MCM7 immunostaining should be largely absent in surface epithelial cells and in most stroma cells.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu MCM7

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Andere Bezeichnung: MCM7 (MCM7 Produkte)

Hintergrund: CDC47, DNA replication licensing factor MCM7, MCM7 mini chromosome maintenance deficient 7, Minichromosome Maintenance 7, Mini chromosome maintenance protein 7, P1.1-MCM3, P1CDC47, P85MCM, PNAS146,MCM7 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P33993

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA