Rekombinanter Nucleolin Antikörper
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- Target Alle Nucleolin (NCL) Antikörper anzeigen
- Nucleolin (NCL)
- Antikörpertyp
- Recombinant Antibody
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Monoklonal
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Konjugat
- Dieser Nucleolin Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (IHC)
- Immunogen
- Recombinant full-length human NCL protein
- Klon
- MSVA-623R
- Isotyp
- IgG
- Top Product
- Discover our top product NCL Primärantikörper
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- Applikationshinweise
- IHC 1:100-1:200
- Kommentare
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Positive Control: Colon: A nuclear nucleolin immunostaining should be seen in all cells. A focus of the staining on nucleoli should be visible - at least - in the superficial epithelial cell layer.
Negative Control: Colon: A cytoplasmic or membranous nucleolin immunostaining should be absent in all cells.
- Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Lagerung
- 4 °C,-20 °C,-80 °C
- Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Target
- Nucleolin (NCL)
- Andere Bezeichnung
- Nucleolin (NCL Produkte)
- Synonyme
- C23 antikoerper, B530004O11Rik antikoerper, D0Nds28 antikoerper, D1Nds28 antikoerper, Nucl antikoerper, wu:fa12d03 antikoerper, zgc:152810 antikoerper, nucl antikoerper, LOC100282923 antikoerper, LOC100283058 antikoerper, nucleolin antikoerper, nucleolin S homeolog antikoerper, NCL antikoerper, Ncl antikoerper, ncl.S antikoerper, ncl antikoerper, LOC100195537 antikoerper, LOC100282923 antikoerper, LOC100283058 antikoerper
- Hintergrund
- NCL, Nucl, Nucleolin, Protein C23,Nucleolin antibody validated for immunohistochemistry on 76 different Normal Tissues
- UniProt
- P19338
- Pathways
- Ribonucleoprotein Complex Subunit Organization
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