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Occludin Antikörper (AA 282-415)

OCLN Reaktivität: Human IHC Wirt: Maus Monoclonal MSVA-415M unconjugated
Produktnummer ABIN7477543
  • Target Alle Occludin (OCLN) Antikörper anzeigen
    Occludin (OCLN)
    Bindungsspezifität
    • 16
    • 16
    • 15
    • 9
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 282-415
    Reaktivität
    • 103
    • 66
    • 47
    • 34
    • 18
    • 13
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    Human
    Wirt
    • 87
    • 17
    • 3
    • 1
    Maus
    Klonalität
    • 86
    • 22
    Monoklonal
    Konjugat
    • 45
    • 9
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser Occludin Antikörper ist unkonjugiert
    Applikation
    • 87
    • 34
    • 28
    • 26
    • 26
    • 15
    • 13
    • 13
    • 11
    • 7
    • 6
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC)
    Immunogen
    ecombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)
    Klon
    MSVA-415M
    Isotyp
    IgG
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  • Applikationshinweise
    IHC 1:100-1:200
    Kommentare

    Positive Control: Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes.

    Negative Control: Colon: All non-epithelial and non-endothelial cells must not show any occludin staining.

    Protokoll
    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Lagerung
    4 °C,-20 °C,-80 °C
    Informationen zur Lagerung
    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • Target
    Occludin (OCLN)
    Andere Bezeichnung
    Occludin (OCLN Produkte)
    Synonyme
    AI503564 antikoerper, Ocl antikoerper, ocln antikoerper, oclnb antikoerper, tpmt antikoerper, OCLN antikoerper, wu:fd23h10 antikoerper, wu:fi13c01 antikoerper, zgc:113992 antikoerper, zgc:56359 antikoerper, BLCPMG antikoerper, occludin antikoerper, occludin S homeolog antikoerper, occludin a antikoerper, Ocln antikoerper, OCLN antikoerper, ocln.S antikoerper, ocln antikoerper, oclna antikoerper
    Hintergrund
    BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PPP1R115, PTORCH1, Tight junction protein occludin,Occludin antibody validated for immunohistochemistry on 76 different Normal Tissues
    UniProt
    Q16625
    Pathways
    Cell-Cell Junction Organization, Hepatitis C
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