S100A12 Antikörper

Produktnummer ABIN7477583

Produktdetails anti-S100A12 Antikörper

Target: S100A12 (S100 Calcium Binding Protein A12 (S100A12))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser S100A12 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-812M

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Spleen: A significant number of inflammatory cells should show a strong S100A12 immunostaining while the majority of cells remain completely negative.

Negative Control: Colon: All epithelial and muscular cells must not show any S100A12 immunostaining. Few scattered S100A12 positive inflammatory cells can occur (mostly within blood vessels)

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu S100A12

Target: S100A12 (S100 Calcium Binding Protein A12 (S100A12))

Andere Bezeichnung: S100A12 (S100A12 Produkte)

Hintergrund: CAAF1, CAGC, CGRP, ENRAGE, MRP6, p6,S100A12 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P80511

Pathways: Toll-Like Receptors Cascades, S100 Proteine