UPK1A Antikörper (AA 114-173)

Produktnummer ABIN7477611

Produktdetails anti-UPK1A Antikörper

Target: UPK1A (Uroplakin 1a (UPK1A))

Bindungsspezifität: AA 114-173

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser UPK1A Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Immunogen: Recombinant fragment (around aa 114-173) of human Uroplakin 1A (UPK1A) protein (exact sequence is proprietary)

Klon: MSVA-735M

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Urinary bladder: A strong membranous and cytoplasmic Upk1a immunostaining should be seen in the urothelium (the staining can be limited to the top 1/3 or 2/3 of the urothelium).

Negative Control: Colon: Upk1a immunostaining should be absent in all cells of the colon mucosa.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu UPK1A

Target: UPK1A (Uroplakin 1a (UPK1A))

Andere Bezeichnung: Uroplakin 1A (UPK1A Produkte)

Hintergrund: UP1A, UPIA, UPKA, TSPAN21,Uroplakin 1A antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: O00322