Uroplakin 1B Antikörper (AA 109-229)

Produktnummer ABIN7477613

Produktdetails anti-Uroplakin 1B Antikörper

Target: Uroplakin 1B (UPK1B)

Bindungsspezifität: AA 109-229

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Uroplakin 1B Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Immunogen: Recombinant fragment (around aa 109-229) of human Uroplakin 1B (UPK1B) protein (exact sequence is proprietary)

Klon: MSVA-734M

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Urinary bladder: A strong membranous and cytoplasmic UPK1b immunostaining should be seen in the urothelium (the staining can be limited to the top cell layers or equally involve all cell layers).

Negative Control: Colon: UPK1b immunostaining should be absent in all cells of the colon mucosa.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu Uroplakin 1B

Target: Uroplakin 1B (UPK1B)

Andere Bezeichnung: Uroplakin 1B (UPK1B Produkte)

Hintergrund: Tetraspanin-20, Tspan-20, TSPAN20, UP1b, UPIb, UPK1B, Uroplakin-1b,Uroplakin 1B antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: O75841