E. Coli Antikörper (FITC)

Produktnummer ABIN7478177

Produktdetails anti-E. Coli Antikörper (FITC)

Target: E. Coli

Reaktivität: E. coli

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser E. Coli Antikörper ist konjugiert mit FITC

Applikation: Functional Studies (Func)

Spezifität: Many "O" and "K" antigenic serotypes of Escherichia coli

Kreuzreaktivität (Details): Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae

Aufreinigung: This product consists of purified IgG fraction of the neat antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

Immunogen: Mixture of E. coli serotypes

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.

Details zu E. Coli

Target: E. Coli

Abstract: E. Coli Produkte

Substanzklasse: Bacteria