Dieser Elementary Bodies Antikörper ist konjugiert mit Peroxidase (POD)
Applikation
Immunohistochemistry (IHC), Western Blotting (WB), Enzyme Immunoassay (EIA)
Spezifität
Purified elementary bodies, disrupted
Kreuzreaktivität (Details)
Cross-reacts with Chlamydia psittacii & Chlamydia pneumoniae (TWAR) UNINFECTED CELL REACTIVITY: Negative vs. HEp-2 cells and egg yolk sac
Aufreinigung
This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
EB
Reaktivität: Chlamydia trachomatis
IF
Wirt: Kaninchen
Polyclonal
Biotin
Applikationshinweise
TITER : 1:1,000-3,000 by indirect immunofluorescence vs. all serovars (A-K, L1-L3), This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
Lot specific
Buffer
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
Konservierungsmittel
Thimerosal (Merthiolate)
Vorsichtsmaßnahmen
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.