This antibody is expressed in HEK293 cells and and was purified using a combination of Protein A/G chromatography. It is reactive against the EV membrane protein of monkeypox virus (Homologous to Vaccinia virus B5R). It also reacts to VACV antigen and lysate, CPXV lysate, MPXV lysate, and VARV antigen and lysate. MPXV-13 weakly binds to VACV purified antigen, VACV and MPXV virus-infected cell lysates, and strongly binds VARV purified antigen and CPXV virus-infected cell lysate. This product is sold for research use only. Optimal working concentrations should be determined by each researcher.
Aufreinigung
This human recombinant IgG1 antibody is purified using a multiple step process to include Protein A or Protein G chromatography. It is produced using in vitro cell culture techniques. Antibody concentration was determined using an absorbance at 280 nm: 1.4 equals 1.0 mg of IgG.
Reinheit
Purified Protein
Klon
1E2
Isotyp
IgG1
Applikationshinweise
This antibody reacts with monkeypox virus as determined by ELISA. Optimal working dilutions should be determined experimentally by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
PBS, pH 7.2, 0.09 % Sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-80 °C
Informationen zur Lagerung
Antibody may be stored between 2°C and 8°C for up to 30 days. For long-term storage, aliquot and store at -80°C. Multiple freeze-thaw cycles are not recommended.