Nuclease Antikörper
Reaktivität: Serratia marcescens
ELISA, WB
Wirt: Kaninchen
Polyclonal
unconjugated
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- Target Alle Nuclease Produkte
- Nuclease
- Reaktivität
- Serratia marcescens
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Nuclease Antikörper ist unkonjugiert
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Applikation
- ELISA, Western Blotting (WB)
- Verwendungszweck
- Nuclease/NucA Antibody
- Kreuzreaktivität (Details)
- This antibody will react with endonuclease from S.
- Aufreinigung
- This antibody was purified from monospecific antiserum by Protein A chromatography.
- Sterilität
- Sterile filtered
- Immunogen
- Anti-Nuclease/NucA was prepared by repeated immunizations with recombinant benzonase protein produced in E. coli.
- Isotyp
- IgG
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- Applikationshinweise
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ELISA_Dilution: 1:21,600 - 1:22,600
Western_Blot_Dilution: 1:1000
- Kommentare
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Anti-Nuclease/NucA has been tested by ELISA and western blot. Expect a band ~ 28 kDa in size corresponding to endonuclease by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Haltbarkeit
- 12 months
-
- Target
- Nuclease
- Andere Bezeichnung
- nucA (Nuclease Produkte)
- Synonyme
- F15D2.37 antikoerper, F15D2_37 antikoerper, 5'-3' exonuclease family protein antikoerper, Nuclease antikoerper, AT1G29630 antikoerper, Nuc antikoerper
- Hintergrund
- Benzonase, Nuclease, Endonuclease, Serratia marcescens endonuclease, NucA,Nucleases are secreted by Serratia marcescens into the medium it surrounds. The enzyme is a sugar-nonspecific hydrolase, capable of cleaving both RNA and DNA in either double or single stranded form. It requires divalent cations, preferably Mg2+, and is functional across a broad pH range from 6 to 10 (optimal at 8-8.5) and wide temperature ranges between 35 °C and 44 °C. The ability of Serratia to secrete nuclease appears to be regulated. Bacterial cultures at differing cell densities display different kinetics and efficiencies of nuclease secretion [i.e. growth medium, growth conditions, and host cell mutations]. Anti-Nuclease/NucA Antibody is useful for researcher interested in identifying nucleic acid contamination.
- UniProt
- P13717
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