Rekombinanter HLA-A2 Antikörper

Produktnummer ABIN7543207

Produktdetails

Target: HLA-A2

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), ELISA

Verwendungszweck: Anti-HLA-A2 [BB7.2], Rabbit IgG, kappa

Spezifität: This antibody binds the carboxy terminus of the α2-helix of class I MHC molecule and a turn on one of the underlying β-strands.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with papain solubilized HLA-A2 and B7 antigens.

Klon: BB7-2

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The binding specificity of BB7.2 towards HLA-A2 was assessed using complement-mediated cytotoxicity and cellular radio binding assay. This antibody is cytotoxic for peripheral blood leukocytes and lymphoid cell lines. The original IgG2b version of this antibody binds HLA-A2 positive cells with a binding or avidity constant of about 5 x 108 liter/mol (PMID:7035415). This antibody was used to detect HLA-A*0201-PR1 complexes using western blot and ELISA (PMID: 17493346). This antibody was also used to detect HLA-A2 complexed with pol, gag, and EBV peptides using ELISA (PMID:8562505). This antibody was used to screen human leucocyte antigen (HLA)-A2 haplotype-positive HBV patients using flow cytometry (PMID: 16108766). This antibody was also used for the immunofluorescent staining of HLA-A2.1 Molecules on the cell surface of human processing defective cell line (T2) (PMID:7684681). This antibody was used in a study involving the expression of HLA class molecules in head and neck squamous cell carcinoma (HNSCC) specimens by immunohistochemistry (PMID: 9328140).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Antigendetails

Target: HLA-A2

Substanzklasse: Influenza Protein

Hintergrund: HLA-A, Human leukocyte antigen A, HLA class I histocompatibility antigen, A-2 alpha chain, MHC class I antigen A-2 HLA-A, HLA class I histocompatibility antigen A-28 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, MHC class I antigen A-28

UniProt: P04439