Rekombinanter XCR1 Antikörper

Produktnummer ABIN7543271

Produktdetails anti-XCR1 Antikörper

Target: XCR1 (Chemokine (C Motif) Receptor 1 (XCR1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser XCR1 Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS)

Verwendungszweck: Anti-XCR1 [5G7], Rabbit IgG, kappa

Spezifität: This antibody binds human XCR1, more particularly it strongly binds amino acids 'PESTTFFYYDLQ' of the human XCR1. XRC1 is a receptor for chemokines SCYC1 and SCYC2 and expressed on 80 % of CD8+ the dendritic cells. It also acts as a receptor for XCL1/Lymphotactin. It transduces a signal by increasing the intracellular calcium ions level.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing XCR1 knockout mice with human XCR1-expressing B300.19 cells mixed with equal volume of GERBU adjuvant.

Klon: 5G7

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody is capable of binding human XCR1-EGFP-expressing B300.19 cells in a flow cytometry analysis. The determination of the binding epitope of this antibody was done using ELISA (US9371389). This antibody exhibits a significant effect in treating rheumatoid arthritis in the experiment of delayed-type hypersensitivity reaction induced by Mycobacterium butyricum delayed-type hypersensitivity reaction. The neutralizing activity of this antibody against human lymphotactin-induced migration of human XCR1-expressing cells was evaluated by in vitro chemotaxis assay. It was reported that this antibody completely inhibited cell migration at a concentration of about 3 μg/mL (US9371389). The effect of this antibody on ear swelling of DNFB (Dinitrofluorobenzene)-sensitized mice was done and it was reported that this antibody caused significant suppression of ear swelling 24 after induction. This antibody was also reported to show showed a very low reactivity to human CX3CR1-EGFP-expressing B300.19 cells (US9371389). The original antibody with saporin-conjugated secondary antibody showed growth suppression of human XCR1-EGFP-expressing B300.19 cells. The humanized version of this antibody is also available on request (US9371389).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu XCR1

Target: XCR1 (Chemokine (C Motif) Receptor 1 (XCR1))

Andere Bezeichnung: XCR1 (XCR1 Produkte)

Hintergrund: CCXCR1, GPR5, chemokine XC receptor 1, G-protein coupled receptor 5, lymphotactin receptor

UniProt: P46094